WEKO3
アイテム
{"_buckets": {"deposit": "8eb3a663-8626-419a-921c-2337fa2af58a"}, "_deposit": {"created_by": 11, "id": "3519", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "3519"}, "status": "published"}, "_oai": {"id": "oai:kagawa-u.repo.nii.ac.jp:00003519", "sets": ["413"]}, "author_link": ["849", "512"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-02-28", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "112", "bibliographicPageEnd": "53", "bibliographicPageStart": "49", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "香川大学農学部学術報告", "bibliographic_titleLang": "ja"}, {"bibliographic_title": "Technical bulletin of Faculty of Agriculture, Kagawa University", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Rapid determination of ploidy levels in cyclamen by using flow cytometry of DAPI‐stained nuclei was examined. A strong positive linear correlation between DAPI fluorescent intensities of nuclei extracted from the leaf blades of cyclamen plants and the ploidy levels in diploid, triploid and tetraploid plants was confirmed. Thus, the flow cyto‐metry of DAPI‐stained nuclei in cyclamen was proven to be an effective and easy tool to determine the ploidy levels within a short time. DAPI fluorescent intensities of nuclei extracted from the pollen grains of diploid plants having and without giant pollen grains were also investigated. Three peaks of 1C, 2C and 4C were observed in the plants having giant pollen grains, whereas only 1C and 2C peaks were observed in the plants without giant pollen. The results suggested that the giant pollen grains were unreduced 2n pollen grains.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}, {"subitem_description": "フローサイトメトリーを用いた、シクラメンの簡易かつ迅速な倍数性検定法の確立を試みた結果、各倍数体レベルとDAPIにより染色された細胞内核DNAの蛍光強度との間に高い相関が認められ、フローサイトメトリーがシクラメンの簡易倍数性検定に有効な手段であることが示された。また、フローサイトメトリーを用いて、巨大花粉を分離しないまたは分離するシクラメン二倍体個体の花粉分析した結果、前者では1Cと2Cのピークが認められたのに対して、後者では1Cと2Cに加えて4Cのピークが認められ、巨大花粉は非還元性の2n花粉であることが強く示唆された。", "subitem_description_language": "ja", "subitem_description_type": "Abstract"}]}, "item_10002_heading_23": {"attribute_name": "見出し", "attribute_value_mlt": [{"subitem_heading_banner_headline": "応用生物科学科", "subitem_heading_headline": "生物資源生産学大講座", "subitem_heading_language": "ja"}, {"subitem_heading_banner_headline": "Department of Applied Biological Science", "subitem_heading_headline": "Chair of Bioresource Production Science", "subitem_heading_language": "en"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "香川大学農学部", "subitem_publisher_language": "ja"}]}, "item_10002_relation_12": {"attribute_name": "論文ID(NAID)", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "110006270667", "subitem_relation_type_select": "NAID"}}]}, "item_10002_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://agriknowledge.affrc.go.jp/RN/2010734707", "subitem_relation_type_select": "URI"}}]}, "item_10002_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00038339", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0368-5128", "subitem_source_identifier_type": "PISSN"}]}, "item_10002_text_25": {"attribute_name": "WAID", "attribute_value_mlt": [{"subitem_text_value": "849"}]}, "item_10002_text_26": {"attribute_name": "KEID", "attribute_value_mlt": [{"subitem_text_value": "1287"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "高村, 武二郎", "creatorNameLang": "ja"}, {"creatorName": "タカムラ, タケジロウ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Takamura, Takejiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "849", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040253257", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000040253257"}, {"nameIdentifier": "40253257", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40253257"}, {"nameIdentifier": "t-take", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/t-take"}]}, {"creatorNames": [{"creatorName": "吉村, 奈津紀", "creatorNameLang": "ja"}, {"creatorName": "ヨシムラ, ナツキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Yoshimura, Natsuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "512", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006391769 ", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006391769 "}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-14"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AN00038339_059_L049.pdf", "filesize": [{"value": "985.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 985600.0, "url": {"label": "AN00038339_059_L049", "objectType": "fulltext", "url": "https://kagawa-u.repo.nii.ac.jp/record/3519/files/AN00038339_059_L049.pdf"}, "version_id": "5b4b9fee-67ac-4fc4-88a9-4de32a042b7f"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "2n pollen", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cyclamen persicum", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DAPI", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "flow cytometry", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "giant pollen", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ploidy", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "フローサイトメトリーを用いたシクラメン植物体および花粉の倍数性検定", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "フローサイトメトリーを用いたシクラメン植物体および花粉の倍数性検定", "subitem_title_language": "ja"}, {"subitem_title": "Determination of ploidy levels in plants amd pollen grains of Cyclamen persicum mill. by using flow cytometry", "subitem_title_language": "en"}, {"subitem_title": "フロー サイトメトリー オ モチイタ シクラメン ショクブツタイ オヨビ カフン ノ バイスウセイ ケンテイ", "subitem_title_language": "ja-Kana"}]}, "item_type_id": "10002", "owner": "11", "path": ["413"], "permalink_uri": "https://kagawa-u.repo.nii.ac.jp/records/3519", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2012-03-27"}, "publish_date": "2012-03-27", "publish_status": "0", "recid": "3519", "relation": {}, "relation_version_is_last": true, "title": ["フローサイトメトリーを用いたシクラメン植物体および花粉の倍数性検定"], "weko_shared_id": -1}
フローサイトメトリーを用いたシクラメン植物体および花粉の倍数性検定
https://kagawa-u.repo.nii.ac.jp/records/3519
https://kagawa-u.repo.nii.ac.jp/records/35190d5cdd29-58aa-4cb3-9b86-8bcd8ceff2a7
名前 / ファイル | ライセンス | アクション |
---|---|---|
AN00038339_059_L049 (985.6 kB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2012-03-27 | |||||||||||
タイトル | ||||||||||||
言語 | ja | |||||||||||
タイトル | フローサイトメトリーを用いたシクラメン植物体および花粉の倍数性検定 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Determination of ploidy levels in plants amd pollen grains of Cyclamen persicum mill. by using flow cytometry | |||||||||||
タイトル | ||||||||||||
言語 | ja-Kana | |||||||||||
タイトル | フロー サイトメトリー オ モチイタ シクラメン ショクブツタイ オヨビ カフン ノ バイスウセイ ケンテイ | |||||||||||
見出し | ||||||||||||
大見出し | 応用生物科学科 | |||||||||||
小見出し | 生物資源生産学大講座 | |||||||||||
言語 | ja | |||||||||||
見出し | ||||||||||||
大見出し | Department of Applied Biological Science | |||||||||||
小見出し | Chair of Bioresource Production Science | |||||||||||
言語 | en | |||||||||||
言語 | ||||||||||||
言語 | jpn | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | departmental bulletin paper | |||||||||||
アクセス権 | ||||||||||||
アクセス権 | open access | |||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
著者 |
高村, 武二郎
× 高村, 武二郎
WEKO
849
× 吉村, 奈津紀 |
|||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Rapid determination of ploidy levels in cyclamen by using flow cytometry of DAPI‐stained nuclei was examined. A strong positive linear correlation between DAPI fluorescent intensities of nuclei extracted from the leaf blades of cyclamen plants and the ploidy levels in diploid, triploid and tetraploid plants was confirmed. Thus, the flow cyto‐metry of DAPI‐stained nuclei in cyclamen was proven to be an effective and easy tool to determine the ploidy levels within a short time. DAPI fluorescent intensities of nuclei extracted from the pollen grains of diploid plants having and without giant pollen grains were also investigated. Three peaks of 1C, 2C and 4C were observed in the plants having giant pollen grains, whereas only 1C and 2C peaks were observed in the plants without giant pollen. The results suggested that the giant pollen grains were unreduced 2n pollen grains. | |||||||||||
言語 | en | |||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | フローサイトメトリーを用いた、シクラメンの簡易かつ迅速な倍数性検定法の確立を試みた結果、各倍数体レベルとDAPIにより染色された細胞内核DNAの蛍光強度との間に高い相関が認められ、フローサイトメトリーがシクラメンの簡易倍数性検定に有効な手段であることが示された。また、フローサイトメトリーを用いて、巨大花粉を分離しないまたは分離するシクラメン二倍体個体の花粉分析した結果、前者では1Cと2Cのピークが認められたのに対して、後者では1Cと2Cに加えて4Cのピークが認められ、巨大花粉は非還元性の2n花粉であることが強く示唆された。 | |||||||||||
言語 | ja | |||||||||||
書誌情報 |
ja : 香川大学農学部学術報告 en : Technical bulletin of Faculty of Agriculture, Kagawa University 巻 59, 号 112, p. 49-53, 発行日 2007-02-28 |
|||||||||||
出版者 | ||||||||||||
言語 | ja | |||||||||||
出版者 | 香川大学農学部 | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 0368-5128 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AN00038339 | |||||||||||
論文ID(NAID) | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | NAID | |||||||||||
関連識別子 | 110006270667 | |||||||||||
関連サイト | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | URI | |||||||||||
関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010734707 | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 2n pollen | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Cyclamen persicum | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | DAPI | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | flow cytometry | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | giant pollen | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | ploidy | |||||||||||
WAID | ||||||||||||
849 | ||||||||||||
KEID | ||||||||||||
1287 |