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Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri) 神崎, 啓亮 コウザキ, ケイスケ Kohzaki, Keisuke 五味, 剣二 ゴミ, ケンジ Gomi, Kenji Yamasaki-Kokudo, Yumiko 山崎, 祐末子 ヤマサキ, ユミコ Yamasaki, Yumiko 小澤, 理香 オザワ, リカ Ozawa, Rika 高林, 純示 タカバヤシ, ジュンジ Takabayashi, Junji 秋光, 和也 アキミツ, カズヤ Akimitsu, Kazuya open access Copyright © 2009 Elsevier GmbH. All rights reserved. Citrus Monoterpene synthase Sabinene We previously isolated two putative monoterpene synthase genes, RlemTPS1 and RlemTPS2, from rough lemon (Citrus jambhiri) and showed that gene expression of RlemTPS2 was induced by microbial attack. The protein product of RlemTPS2 was obtained using a prokaryotic expression system, and GC and GC-MS of monoterpene synthesis by RlemTPS2 determined that RlemTPS2 encodes a sabinene synthase. Sabinene has antifungal activity toward Alternaria alternata. Furthermore, site-directed mutagenesis identified one amino acid, Ile, located at the front of the metal ion binding motif as an important residue for the product specificity of sabinene synthase. Elsevier 2009-10-15 eng journal article AM https://kagawa-u.repo.nii.ac.jp/records/507 120007015444 19433341 10.1016/j.jplph.2009.04.003 AA10522948 0176-1617 Journal of plant physiology 166 15 1700 1704 https://kagawa-u.repo.nii.ac.jp/record/507/files/AA10522948_166_15_1700.pdf application/pdf 239.1 kB 2020-11-26