2026-03-11T22:14:48Z https://kagawa-u.repo.nii.ac.jp/oai

oai:kagawa-u.repo.nii.ac.jp:00000366 2024-10-31T07:59:51Z 13:40

Acetaldehyde administration induces salsolinol formation in vivo in the dorsal striatum of Aldh2-knockout and C57BL/6N mice Ito, Asuka 伊藤, 明日香 イトウ, アスカ Acetaldehyde Salsolinol Striatum Microdialysis Aldh2-KO mice 香川大学 Kagawa University 博士（医学） Abstract Acetaldehyde (AcH) and salsolinol play important roles in the central effects of ethanol. This study aimed to investigate the effect of administration of AcH on dopamine (DA), DA-derived salsolinol and serotonin (5-HT) levels in the dorsal striatum of Aldh2-knockout (Aldh2-KO) and C57BL/6 N (WT) mice. Animals were treated with AcH (50, 100 and 200 mg/kg) intraperitoneally and dialysate levels of DA, 5-HT and salsolinol were determined using in vivo microdialysis coupled with HPLC-ECD. Salsolinol was first detected at 20 min after AcH administration, and reached its peak concentration (WT mice: 0.29 ± 0.22 pg/μl; Aldh2-KO mice: 0.63 ± 0.17 pg/μl) at 25 min in the 200 mg/kg AcH group, before decreasing rapidly and reaching zero at approximately 55–80 min. Treatment with 100 and 200 mg/kg AcH increased levels of salsolinol in both WT and Aldh2-KO mice, with 200 mg/kg AcH inducing a higher level of salsolinol in Aldh2-KO mice than in WT mice. Treatment with 50 mg/kg AcH produced a small increase in salsolinol levels in Aldh2-KO mice, whereas no elevation of salsolinol was detected in WT mice. The increase in salsolinol formation was found to occur a dose-dependent manner in both genotypes. Administration of AcH and the subsequent changes in salsolinol concentrations did not change DA or 5-HT levels in either genotype. Our study suggests that AcH dose-dependently increases the formation of salsolinol in the dorsal striatum of mice, which provides further support for the role of AcH in salsolinol formation in the animal brain. doctoral thesis 2019-03-24 P application/pdf application/pdf application/pdf 甲第716号 https://kagawa-u.repo.nii.ac.jp/record/366/files/Med_A716.pdf https://kagawa-u.repo.nii.ac.jp/record/366/files/Med_A716_abstract.pdf https://kagawa-u.repo.nii.ac.jp/record/366/files/Med_A716_result.pdf https://kagawa-u.repo.nii.ac.jp/records/366 eng https://doi.org/10.1016/j.neulet.2018.07.032 https://dl.ndl.go.jp/pid/11288049 30036570 500001345906 Copyright © 2018 Elsevier B.V. All rights reserved. この博士論文の本文については、次のエルゼビアの著作権ポリシーの規定により公開しています。「博士論文の場合は、エンバーゴ期間に関係なく機関リポジトリに出版社版を公開することができます。」 The text of this doctoral dissertation is published according to the following Elsevier copyright policy. "In the case of doctoral dissertations, publishers can be published in institutional repositories regardless of the Embargo period." open access