{"created":"2023-05-15T09:48:41.104763+00:00","id":11576,"links":{},"metadata":{"_buckets":{"deposit":"0aaf2dd5-e1c5-4e05-be09-2e4e5a84d592"},"_deposit":{"created_by":11,"id":"11576","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"11576"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00011576","sets":["13:40"]},"author_link":["3101"],"control_number":"11576","item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"形質細胞様樹状細胞によるサイトカイン産生に対するD-アロースの免疫調整効果","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-12-27"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"D-Allose is classified as a 'rare sugar,' i.e., part of the group of monosaccharides that are present in low quantities in the natural world. D-Allose has been demonstrated to exert many physiological functions. The effects of the rare sugars on immune responses are largely unexplored. Here, we investigated the physiological effects of D-allose on murine dendritic cells' cytokine production. When plasmacytoid dendritic cells (pDCs) were stimulated with a Toll-like receptor 7 (TLR7) ligand, a single-stranded RNA (ssRNA), or a TLR9 ligand, CpG DNA, in the medium containing D-allose, the productions of both interferon-alpha (IFN-α) and interleukin (IL)-12p40 were severely decreased. In contrast, a normal production of these cytokines was observed when pDCs were stimulated with other TLR7 ligands, an imidazoquinoline, or a guanosine analog. In contrast to the pDCs, conventional dendritic cells (cDCs) produced IL-12p40 and tumor necrosis factor-alpha (TNF-α) in response to an imidazoquinoline or CpG DNA even though D-allose was present in the medium. D-Allose did not induce pDC death, and not inhibit the endocytic uptake of fluorophore-labeled CpG DNA into pDCs. These results suggested that D-allose exerts its inhibitory effects after CpG DNA is internalized. We analyzed the TLR7/9 signal-induced activation of downstream signaling molecules in pDCs and observed that when pDCs were stimulated with a ssRNA or CpG DNA, the phosphorylation status of the MAPK family, which includes Erk1/2, JNK/SAPK, and p38 MAPK, was attenuated in the presence of D-allose compared to D-glucose controls. The stimulation of pDCs with an imidazoquinoline induced a strong phosphorylation of these MAPK family members even in the presence of D-allose. These findings reveal that D-allose can inhibit the cytokine production by pDCs stimulated with ssRNA or CpG DNA via an attenuation of the phosphorylation of MAPK family members.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第816号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2022.08.037","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/12691034","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"36030654","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001550938","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 Elsevier Inc. All rights reserved.","subitem_rights_language":"en"},{"subitem_rights":"This work was supported by a grant from the Japan Society for the Promotion of Science (JSPS) KAKENHI, no. JP21K08458. This work was also supported by the Program for Building Regional Innovation Ecosystems of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan; the Hyakujushi Bank Foundation for the Promotion of Science and Culture; the Fund for Kagawa University Research Promotion Program; and a Rare Sugar research grant funded by Kagawa Prefecture.","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_1689051968263":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"21K08458","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K08458/"},"subitem_award_titles":[{"subitem_award_title":"形質細胞様樹状細胞の活性を制御する機能性単糖の開発","subitem_award_title_language":"ja"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001700","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"文部科学省 : 地域イノベーション・エコシステム形成プログラム","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Ministry of Education, Culture, Sports, Science and Technology : Program for Building Regional Innovation Ecosystems","subitem_funder_name_language":"en"}]},{"subitem_funder_names":[{"subitem_funder_name":"百十四銀行学術文化振興財団","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Hyakujushi Bank Foundation for the Promotion of Science and Culture","subitem_funder_name_language":"en"}]},{"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/100015075","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"香川大学","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Kagawa University","subitem_funder_name_language":"en"}]},{"subitem_funder_identifiers":{"subitem_funder_identifier":"https://isni.org/isni/0000000404064650","subitem_funder_identifier_type":"ISNI"},"subitem_funder_names":[{"subitem_funder_name":"香川県","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Kagawa Prefecture","subitem_funder_name_language":"en"}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"髙尾, 健二郎","creatorNameLang":"ja"},{"creatorName":"タカオ, ケンジロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Takao, Kenjiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-03"}],"displaytype":"detail","filename":"Med_A816.pdf","filesize":[{"value":"421.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/11576/files/Med_A816.pdf"},"version_id":"4d522eb0-d364-4a3b-b391-c98790c09739"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-03"}],"displaytype":"detail","filename":"Med_A816_abstract.pdf","filesize":[{"value":"77.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/11576/files/Med_A816_abstract.pdf"},"version_id":"983820c1-0d31-4d0a-a557-6152e5e56fe4"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-03"}],"displaytype":"detail","filename":"Med_A816_result.pdf","filesize":[{"value":"200.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/11576/files/Med_A816_result.pdf"},"version_id":"b2e399f6-8a6c-4b6b-aa08-1d46a7ab614f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Plasmacytoid dendritic cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interferon-α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"D-allose","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rare sugar","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MAPK","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Immunomodulatory effects of D-allose on cytokine production by plasmacytoid dendritic cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Immunomodulatory effects of D-allose on cytokine production by plasmacytoid dendritic cells","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-03"},"publish_date":"2023-02-03","publish_status":"0","recid":"11576","relation_version_is_last":true,"title":["Immunomodulatory effects of D-allose on cytokine production by plasmacytoid dendritic cells"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-11T07:05:08.543849+00:00"}