{"created":"2023-05-15T09:48:41.238970+00:00","id":11579,"links":{},"metadata":{"_buckets":{"deposit":"b176c778-dd71-442b-9bbb-c29d8f74dbf7"},"_deposit":{"created_by":11,"id":"11579","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"11579"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00011579","sets":["13:40"]},"author_link":["3104"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"肝細胞癌の細胞周期とマイクロRNAを介したレンバチニブの治療効果の検討","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-12-27"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lenvatinib is a tyrosine kinase receptor inhibitor used to treat unresectable hepatocellular carcinoma (HCC). In this study, we investigated the antitumor effects of Lenvatinib treatment on HCC cell lines. Proliferation was examined in four HCC cell lines (HuH‑7, Hep3B, Li‑7, and PLC/PRF/5) using Cell Counting Kit‑8 assays. Xenograft mouse models were used to assess the effects of Lenvatinib in vivo. Cell cycle, western blotting, and microRNA (miRNA) expression analyses were performed to identify the antitumor inhibitory potential of Lenvatinib on HCC cells. Lenvatinib treatment suppressed proliferation of HuH‑7 and Hep3B, but not Li‑7 and PLC/PRF/5 cells and induced G0/G1 cell cycle arrest and cyclin D1 downregulation in Lenvatinib‑sensitive cells. Lenvatinib treatment also reduced tumor growth in HuH‑7 xenograft mouse models. miRNA microarrays revealed that Lenvatinib treatment altered the expression of miRNAs in HuH7 cells and exosomes. Our results demonstrated the therapeutic potential of Lenvatinib and provide molecular mechanistic insights into its antitumor effects for treating HCC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第819号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3892/br.2022.1561","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc9468852/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"36158319","subitem_relation_type_select":"PMID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Nakahara et al.","subitem_rights_language":"en"},{"subitem_rights":"This is an open access article distributed under the terms of Creative Commons Attribution License.","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中原, 麻衣","creatorNameLang":"ja"},{"creatorName":"ナカハラ, マイ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakahara, Mai","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-03"}],"displaytype":"detail","filename":"Med_A819.pdf","filesize":[{"value":"521.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/11579/files/Med_A819.pdf"},"version_id":"2db79201-aa50-442d-9ff2-bfbd2bb3cdaa"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-03"}],"displaytype":"detail","filename":"Med_A819_abstract.pdf","filesize":[{"value":"85.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/11579/files/Med_A819_abstract.pdf"},"version_id":"5a9a6080-a744-42f1-aed1-6ac0148ceeb8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-03"}],"displaytype":"detail","filename":"Med_A819_result.pdf","filesize":[{"value":"152.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/11579/files/Med_A819_result.pdf"},"version_id":"318a3496-0fc0-4a38-a0e6-7827e090b5e4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Lenvatinib","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell cycle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cyclin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocellular carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effect of Lenvatinib treatment on the cell cycle and microRNA profile in hepatocellular carcinoma cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of Lenvatinib treatment on the cell cycle and microRNA profile in hepatocellular carcinoma cells","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-03"},"publish_date":"2023-02-03","publish_status":"0","recid":"11579","relation_version_is_last":true,"title":["Effect of Lenvatinib treatment on the cell cycle and microRNA profile in hepatocellular carcinoma cells"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-16T02:32:34.782588+00:00"}