@article{oai:kagawa-u.repo.nii.ac.jp:00011587,
 author = {角, 明里咲 and Kado, Arisa and 冨本, 卓 and Tomimoto, Suguru and 川村, 理 and Kawamura, Osamu},
 issue = {128},
 journal = {香川大学農学部学術報告, Technical bulletin of Faculty of Agriculture, Kagawa University},
 month = {Feb},
 note = {Nivalenol (NIV) and deoxynivalenol (DON) are typical B-type trichothecene mycotoxins, which frequently contaminate domestic wheat and barley, causing health hazards to humans and livestock. Many monoclonal antibodies (mAbs) against DON have been developed, and screening methods for DON in cereals by ELISA have been reported using these mAbs. However, there are no reports of the production of a specific antibody mAb against NIV. Therefore, NIV-oxime was prepared by oxime of carbonyl at the 8-position and bound to cationized bovine serum albumin (cBSA).We performed cell fusion of spleen cells from the mouse, which was immunized four times with NIV-oxime-cBSA, and succeeded in establishing NIV specific mAb producing hybridomas for the first time. Our NIV.9 mAb did not cross-reacted with NIV related compounds. NIV. 10, 12, and 13 mAbs weakly cross-reacted with15-acetly DON. NIV.11 mAb cross-reacted with 15-acetly DON, and weakly cross-reacted with DON and 3-acetly DON. When NIV.10 and 11 mAbs with the highest NIV reactivity were used, 1 ng/mL of NIV could be detected in the optimized indirect competitive ELISA. We expect that a simple screening method for NIV in crops will be established by ELISA using these antibodies., NIV-オキシム化蛋白質結合体を作製し、国内産の小麦や大麦を高頻度に汚染しているニバレノール（nivalenol,NIV）に対するモノクローナル抗体（mAb）は初めて作製した。NIV-オキシム-cBSAで免疫したマウス脾臓細胞を細胞融合し、19クローンの抗NIVmAb産生ハイブリドーマを得た。NIV.9 抗体はNIVに特異的に反応した。NIV.10、12と13 抗体は15-acetly DONに弱い交差反応を示した。NIV.11抗体は15-acetly DONに強い交差反応を示し、15-acetly DONとDONに弱い交差反応を示した。NIV.9とNIV.11抗体を用いた競合ELISAでは、1 ng/mLまでのNIVの検出が可能であった。},
 pages = {39--44},
 title = {ニバレノールに対するモノクローナル抗体の作製},
 volume = {75},
 year = {2023},
 yomi = {カド, アリサ and トミモト, スグル and カワムラ, オサム}
}