{"created":"2023-05-15T09:48:47.624282+00:00","id":11724,"links":{},"metadata":{"_buckets":{"deposit":"80fe33fc-1c98-4cf6-b816-222e27fe3710"},"_deposit":{"created_by":11,"id":"11724","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"11724"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00011724","sets":["13:40"]},"author_link":["4112"],"control_number":"11724","item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"希少糖D-allose経口投与による膀胱癌に対する抗腫瘍効果","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"D-allose is a rare sugar that has been reported to up-regulate thioredoxin-interacting protein (TXNIP) expression and affect the production of intracellular reactive oxygen species (ROS). However, the antitumor effect of D-allose is unknown. This study aimed to determine whether orally administered D-allose could be a candidate drug against bladder cancer (BC). To this end, BC cell lines were treated with varying concentrations of D-allose (10, 25, and 50 mM). Cell viability and intracellular ROS levels were assessed using cell viability assay and flow cytometry. TXNIP expression was evaluated using Western blotting. The antitumor effect of orally administered D-allose was assessed using a xenograft mouse model. D-allose reduced cell viability and induced intracellular ROS production in BC cells. Moreover, D-allose stimulated TXNIP expression in a dose-dependent manner. Co-treatment of D-allose and the antioxidant L-glutathione canceled the D-allose-induced reduction in cell viability and intracellular ROS elevation. Furthermore, oral administration of D-allose inhibited tumor growth without adverse effects (p < 0.05). Histopathological findings in tumor tissues showed that D-allose decreased the nuclear fission rate from 4.1 to 1.1% (p = 0.004). Oral administration of D-allose suppressed BC growth in a preclinical mouse model, possibly through up-regulation of TXNIP expression followed by an increase in intracellular ROS. Therefore, D-allose is a potential therapeutic compound for the treatment of BC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第827号"}]},"item_10006_link_24":{"attribute_name":"PubMed Central","attribute_value_mlt":[{"subitem_link_text":"PMC9224251","subitem_link_url":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc9224251/"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms23126771","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/12864279/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"35743212","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001563429","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 by the authors. Licensee MDPI, Basel, Switzerland.","subitem_rights_language":"en"},{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"},{"subitem_rights":"This research was funded by the Regional Innovation Ecosystems of the Ministry of Education, Culture, Sports, Science, and Technology, Japan and JSPS KAKENHI (Grant Number: JP20K09559).","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_1689051968263":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"20K09559","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K09559/"},"subitem_award_titles":[{"subitem_award_title":"希少糖D-alloseを用いた新規腎細胞癌治療の構築：抗腫瘍メカニズムの解明","subitem_award_title_language":"ja"},{"subitem_award_title":"Development of a novel treatment strategy for renal cell carcinoma using the rare sugar D-allose: Elucidation of the antitumor mechanism","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"土肥, 洋一郎","creatorNameLang":"ja"},{"creatorName":"トヒ, ヨウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Tohi, Yoichiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-24"}],"displaytype":"detail","filename":"Med_A827.pdf","filesize":[{"value":"2.6 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kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/11724/files/Med_A827_result.pdf"},"version_id":"ae7acf4e-c7fc-481a-8926-cd1be3095164"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"bladder cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"D-allose","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rare sugar","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"reactive oxygen species","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"thioredoxin-interacting protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Antitumor effects of orally administered rare sugar D-Allose in bladder cancer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Antitumor effects of orally administered rare sugar D-Allose in bladder cancer","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-24"},"publish_date":"2023-04-24","publish_status":"0","recid":"11724","relation_version_is_last":true,"title":["Antitumor effects of orally administered rare sugar D-Allose in bladder cancer"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-05T07:02:26.515057+00:00"}