{"created":"2023-05-15T09:48:47.944976+00:00","id":11731,"links":{},"metadata":{"_buckets":{"deposit":"19901151-f628-422e-b3f9-3e1f0f7ba4d1"},"_deposit":{"created_by":11,"id":"11731","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"11731"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00011731","sets":["13:40"]},"author_link":["4119"],"control_number":"11731","item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"脳虚血の ex vivo マウスモデルにおける細胞質型ホスホリパーゼA₂イプシロンによるN-アシル-ホスファチジルエタノールアミンの生成","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"N-Acyl-phosphatidylethanolamines (NAPEs), a minor class of membrane glycerophospholipids, accumulate along with their bioactive metabolites, N-acylethanolamines (NAEs) during ischemia. NAPEs can be formed through N-acylation of phosphatidylethanolamine by cytosolic phospholipase A2ε (cPLA2ε, also known as PLA2G4E) or members of the phospholipase A and acyltransferase (PLAAT) family. However, the enzyme responsible for the NAPE production in brain ischemia has not yet been clarified. Here, we investigated a possible role of cPLA2ε using cPLA2ε-deficient (Pla2g4e−/−) mice. As analyzed with brain homogenates of wild-type mice, the age dependency of Ca2+-dependent NAPE-forming activity showed a bell-shape pattern being the highest at the first week of postnatal life, and the activity was completely abolished in Pla2g4e−/− mice. However, liquid chromatography-tandem mass spectrometry revealed that the NAPE levels of normal brain were similar between wild-type and Pla2g4e−/− mice. In contrast, post-mortal accumulations of NAPEs and most species of NAEs were only observed in decapitated brains of wild-type mice. These results suggested that cPLA2ε is responsible for Ca2+-dependent formation of NAPEs in the brain as well as the accumulation of NAPEs and NAEs during ischemia, while other enzyme(s) appeared to be involved in the maintenance of basal NAPE levels.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第834号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbalip.2022.159222","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/12864149/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"35988872","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001563307","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 Elsevier B.V. All rights reserved.","subitem_rights_language":"en"},{"subitem_rights":"This work was supported by Grants-in-Aid for Scientific Research (JP19K23828 to Z.H., JP20H05691 to M.M., and JP19K07353 to N.U.) from the Japan Society for the Promotion of Science; Strategic Research Support Fund of Kagawa University Research Promotion Program 2021 (KURPP) (to T.U.); Charitable Trust MIU Foundation Memorial Fund (to T.U.); Takeda Science Foundation (to T.U.); and AMED-CREST JP22gm1210013 (to M.M.) from the Japan Agency for Medical Research and Development.","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_1689051968263":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"19K23828","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K23828/"},"subitem_award_titles":[{"subitem_award_title":"Investigation of the neuroprotective effect of the rare phospholipid NAPE using gene-deficient mice","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"20H05691","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H05691/"},"subitem_award_titles":[{"subitem_award_title":"脂質代謝酵素PLA2ファミリーを基軸とした脂質による生命応答制御の統一的理解","subitem_award_title_language":"ja"},{"subitem_award_title":"Regulatory roles of bioactive lipids driven by the phospholipase A2 family","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"19K07353","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K07353/"},"subitem_award_titles":[{"subitem_award_title":"トリアシル型リン脂質の生理機能解析","subitem_award_title_language":"ja"},{"subitem_award_title":"The analysis of physiological functions of triacyl-type phospholipids","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_award_titles":[{"subitem_award_title":"肥満誘導時における脂質代謝酵素群PLAATの生理機能解析","subitem_award_title_language":"ja"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/100015075","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"香川大学研究推進事業 戦略的研究支援経費","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Strategic Research Support Fund of Kagawa University Research Promotion Program","subitem_funder_name_language":"en"}]},{"subitem_award_titles":[{"subitem_award_title":"脳虚血時に著増する生理活性脂質の機能解析","subitem_award_title_language":"ja"}],"subitem_funder_names":[{"subitem_funder_name":"公益信託エム・アイ・ユー財団記念基金","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Charitable Trust MIU Foundation Memorial Fund","subitem_funder_name_language":"en"}]},{"subitem_award_titles":[{"subitem_award_title":"新奇脂質代謝酵素PLAATファミリーが示す生体内機能の包括的な解析","subitem_award_title_language":"ja"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/100007449","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"武田科学振興財団","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Takeda Science Foundation","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"22gm1210013h0003"},"subitem_award_titles":[{"subitem_award_title":"疾患脂質代謝に基づく生体組織の適応・修復機構の新基軸の創成と医療技術シーズの創出","subitem_award_title_language":"ja"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/100009619","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本医療研究開発機構","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Agency for Medical Research and Development","subitem_funder_name_language":"en"}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Rahman, S.M. Khaledur","creatorNameLang":"ja"},{"creatorName":"ラハマン, エス エム カレドゥル","creatorNameLang":"ja-Kana"},{"creatorName":"Rahman, S.M. Khaledur","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-25"}],"displaytype":"detail","filename":"Med_A834.pdf","filesize":[{"value":"5.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/11731/files/Med_A834.pdf"},"version_id":"e296f070-92e0-40f4-8e1e-d693bfaebc20"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-25"}],"displaytype":"detail","filename":"Med_A834_abstract.pdf","filesize":[{"value":"67.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/11731/files/Med_A834_abstract.pdf"},"version_id":"736094ef-c889-4c9f-b078-af299901e834"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-25"}],"displaytype":"detail","filename":"Med_A834_result.pdf","filesize":[{"value":"63.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/11731/files/Med_A834_result.pdf"},"version_id":"380c3c96-1fac-4bf1-b11a-a3377a5a3d7e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"N-acylethanolamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"N-acyl-phosphatidylethanolamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"N-acyltransferase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Brain ischemia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endocannabinoid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipase A2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Formation of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A₂ε in an ex vivo murine model of brain ischemia","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Formation of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A₂ε in an ex vivo murine model of brain ischemia","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-25"},"publish_date":"2023-04-25","publish_status":"0","recid":"11731","relation_version_is_last":true,"title":["Formation of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A₂ε in an ex vivo murine model of brain ischemia"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-03-05T08:04:57.769945+00:00"}