{"created":"2023-05-15T09:41:08.108889+00:00","id":146,"links":{},"metadata":{"_buckets":{"deposit":"01068ba6-56df-4924-aba6-38e34d6ffd35"},"_deposit":{"created_by":11,"id":"146","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"146"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000146","sets":["13:40"]},"author_link":["3140"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"転写因子Spi-BによるInterferon-α4遺伝子の転写活性化メカニズム","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-06-25"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Plasmacytoid dendritic cells (pDCs) are characterized by an exclusive expression of nucleic acid sensing Toll-like receptor 7 (TLR7) and TLR9, and production of high amounts of type I interferon (IFN) in response to TLR7/9 signaling. This function is crucial for both antiviral immunity and the pathogenesis of autoimmune diseases. An Ets family transcription factor, i.e., Spi-B (which is highly expressed in pDCs) is required for TLR7/9 signal-induced type I IFN production and can transactivate IFN-α promoter in synergy with IFN regulatory factor-7 (IRF-7). Herein, we analyzed how Spi-B contributes to the transactivation of the Ifna4 promoter. We performed deletion and/or mutational analyses of the Ifna4 promoter and an electrophoretic mobility shift assay (EMSA) and observed an Spi-B binding site in close proximity to the IRF-7 binding site. The EMSA results also showed that the binding of Spi-B to the double-stranded DNA probe potentiated the recruitment of IRF-7 to its binding site. We also observed that the association of Spi-B with transcriptional coactivator p300 was required for the Spi-B-induced synergistic enhancement of the Ifna4 promoter activity by Spi-B. These results clarify the molecular mechanism of action of Spi-B in the transcriptional activation of the Ifna4 promoter.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第750号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2020.02.101","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/11526078","subitem_relation_type_select":"URI"}}]},"item_10006_relation_16":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"This is the Publisher's Version of the following article: The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene; Biochemical and biophysical research communications; Volume 525 Issue 2 (2020) Pages:477-482; doi: 10.1016/j.bbrc.2020.02.101, which has been published in final form at https://doi.org/10.1016/j.bbrc.2020.02.101."}]}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"32111355","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001398870","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2020 Elsevier Inc.","subitem_rights_language":"en"},{"subitem_rights":"この博士論文の本文については、次のエルゼビアの著作権ポリシーの規定により公開しています。「博士論文の場合は、エンバーゴ期間に関係なく機関リポジトリに出版社版を公開することができます。」","subitem_rights_language":"ja"},{"subitem_rights":"The text of this doctoral dissertation is published according to the following Elsevier copyright policy. \"In the case of doctoral dissertations, publishers can be published in institutional repositories regardless of the Embargo period.\"","subitem_rights_language":"en"}]},"item_10006_text_30":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"29000"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Miyazaki, Ryo ","creatorNameLang":"en"},{"creatorName":"宮嵜, 亮","creatorNameLang":"ja"},{"creatorName":"ミヤザキ, リョウ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-09"}],"displaytype":"detail","filename":"Med_A750.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/146/files/Med_A750.pdf"},"version_id":"ece0f6a0-c840-499c-a1e1-30dbf87e5c96"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-09"}],"displaytype":"detail","filename":"Med_A750_abstract.pdf","filesize":[{"value":"78.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/146/files/Med_A750_abstract.pdf"},"version_id":"80d4d87e-a852-4268-a5ee-bd826eb03c08"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-09"}],"displaytype":"detail","filename":"Med_A750_result.pdf","filesize":[{"value":"77.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/146/files/Med_A750_result.pdf"},"version_id":"612a24eb-7dc2-4a1c-839b-92f75bce1a76"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"IRF-7","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interferon-α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmacytoid dendritic cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Spi-B","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"p300","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-07-10"},"publish_date":"2020-07-10","publish_status":"0","recid":"146","relation_version_is_last":true,"title":["The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-02-09T00:59:22.804374+00:00"}