{"created":"2023-09-28T08:42:16.201954+00:00","id":2000048,"links":{},"metadata":{"_buckets":{"deposit":"193b0fa8-9bbd-4e0a-80a5-bf5b1ba15db1"},"_deposit":{"created_by":11,"id":"2000048","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000048"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:02000048","sets":["13:40"]},"author_link":["4134"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-09-28"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Histopathological changes occur in the brainstem during the early stages of Alzheimer's disease (AD), with the pathological changes of the brain lesions ascending progressively in accordance with the Braak staging system. The senescence‑accelerated mouse prone 8 (SAMP8) mouse model has been previously used as a model of age‑dependent neurodegenerative diseases, including AD. In the present study, microRNAs (miRNAs) that were upregulated or downregulated in SAMP8 brainstems were identified using miRNA profiling of samples obtained from miRNA arrays. The preliminary stage of cognitive dysfunction was examined using male 5‑month‑old SAMP8 mice, with age‑matched senescence‑accelerated mouse resistant 1 mice as controls. A Y‑maze alternation test was performed to assess short‑term working memory and miRNA profiling was performed in each region of the dissected brain (brainstem, hippocampus and cerebral cortex). SAMP8 mice tended to be hyperactive, but short‑term working memory was preserved. Two miRNAs were upregulated (miR‑491‑5p and miR‑764‑5p) and two were downregulated (miR‑30e‑3p and miR‑323‑3p) in SAMP8 brainstems. In SAMP8 mice, the expression level of upregulated miRNAs were the highest in the brainstem, wherein age‑related brain degeneration occurs early. It was demonstrated that the order of specific miRNA expression levels corresponded to the progression order of age‑related brain degeneration. Differentially expressed miRNAs regulate multiple processes, including neuronal cell death and neuron formation. Changes in miRNA expression may result in the induction of target proteins during the early stages of neurodegeneration in the brainstem. These findings suggest that studying altered miRNA expression may provide molecular evidence for early age‑related neuropathological changes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第841号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3892/mmr.2023.13032","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308455/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"37326032","subitem_relation_type_select":"PMID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: © Kawakita et al. This is an open access article distributed under the terms of Creative Commons Attribution License.","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_1689051968263":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"22K17828","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K17828/"},"subitem_award_titles":[{"subitem_award_title":"脳の老化性変化を誘導するmicroRNAの同定","subitem_award_title_language":"ja"},{"subitem_award_title":"Identification of microRNAs that induce senescent changes in the brain","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawakita, Rie","creatorNameLang":"en"},{"creatorName":"カワキタ, リエ","creatorNameLang":"ja-Kana"},{"creatorName":"川北, 梨愛","creatorNameLang":"ja"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-28"}],"filename":"Med_A841.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/2000048/files/Med_A841.pdf"},"version_id":"a10abcf2-eb21-4140-90d2-641a946243ca"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-28"}],"filename":"Med_A841_abstract.pdf","filesize":[{"value":"461 KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/2000048/files/Med_A841_abstract.pdf"},"version_id":"ece25169-a714-46a8-958f-d7dee67a8b20"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-28"}],"filename":"Med_A841_result.pdf","filesize":[{"value":"104 KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/2000048/files/Med_A841_result.pdf"},"version_id":"83eb26ba-910d-4f6d-b6ae-43507aba26a5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SAMP8 mouse","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"aging","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"brainstem","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"neurodegeneration","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Age‑related brainstem degeneration through microRNA modulation in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Age‑related brainstem degeneration through microRNA modulation in mice","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-09-28"},"publish_date":"2023-09-28","publish_status":"0","recid":"2000048","relation_version_is_last":true,"title":["Age‑related brainstem degeneration through microRNA modulation in mice"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-16T02:33:20.916643+00:00"}