{"created":"2024-08-05T09:56:14.739170+00:00","id":2000516,"links":{},"metadata":{"_buckets":{"deposit":"5c62ad70-0921-4783-9f43-a3367ac4a430"},"_deposit":{"created_by":11,"id":"2000516","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000516"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:02000516","sets":["2"]},"author_link":["4403","4404","4405"],"control_number":"2000516","item_10001_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicVolumeNumber":"298","bibliographic_titles":[{"bibliographic_title":"Journal of biological chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Biological membranes are composed of a wide variety of lipids. Phosphoinositides (PIPns) in the membrane inner leaflet only account for a small percentage of the total membrane lipids but modulate the functions of various membrane proteins, including ion channels, which play important roles in cell signaling. KcsA, a prototypical K+ channel that is small, simple, and easy to handle, has been broadly examined regarding its crystallography, in silico molecular analysis, and electrophysiology. It has been reported that KcsA activity is regulated by membrane phospholipids, such as phosphatidylglycerol. However, there has been no quantitative analysis of the correlation between direct lipid binding and the functional modification of KcsA, and it is unknown whether PIPns modulate KcsA function. Here, using contact bubble bilayer recording, we observed that the open probability of KcsA increased significantly (from about 10% to 90%) when the membrane inner leaflet contained only a small percentage of PIPns. In addition, we found an increase in the electrophysiological activity of KcsA correlated with a larger number of negative charges on PIPns. We further analyzed the affinity of the direct interaction between PIPns and KcsA using microscale thermophoresis and observed a strong correlation between direct lipid binding and the functional modification of KcsA. In conclusion, our approach was able to reconstruct the direct modification of KcsA by PIPns, and we propose that it can also be applied to elucidate the mechanism of modification of other ion channels by PIPns.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology","subitem_publisher_language":"en"},{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_relation_13":{"attribute_name":"item_10001_relation_13","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"35839854","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jbc.2022.102257","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc9396063/","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 The Authors.","subitem_rights_language":"en"},{"subitem_rights":"This is an open access article under the CC BY license.","subitem_rights_language":"en","subitem_rights_resource":"http://creativecommons.org/licenses/by/4.0/"}]},"item_10001_source_id_11":{"attribute_name":"item_10001_source_id_11","attribute_value_mlt":[{"subitem_source_identifier":"AA1202441X","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"item_10001_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"1083-351X","subitem_source_identifier_type":"EISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"紀谷, 拓音","creatorNameLang":"ja"},{"creatorName":"キヤ, タクナリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kiya, Takunari","creatorNameLang":"en"}],"familyNames":[{"familyName":"紀谷","familyNameLang":"ja"},{"familyName":"キヤ","familyNameLang":"ja-Kana"},{"familyName":"Kiya","familyNameLang":"en"}],"givenNames":[{"givenName":"拓音","givenNameLang":"ja"},{"givenName":"タクナリ","givenNameLang":"ja-Kana"},{"givenName":"Takunari","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4403","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"川鍋, 陽","creatorNameLang":"ja"},{"creatorName":"カワナベ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawanabe, Akira","creatorNameLang":"en"}],"familyNames":[{"familyName":"川鍋","familyNameLang":"ja"},{"familyName":"カワナベ","familyNameLang":"ja-Kana"},{"familyName":"Kawanabe","familyNameLang":"en"}],"givenNames":[{"givenName":"陽","givenNameLang":"ja"},{"givenName":"アキラ","givenNameLang":"ja-Kana"},{"givenName":"Akira","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4404","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010707128","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000010707128"},{"nameIdentifier":"10707128","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10707128"},{"nameIdentifier":"silverstone","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/silverstone"},{"nameIdentifier":"0000-0002-1094-6209","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-1094-6209"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"藤原, 祐一郎","creatorNameLang":"ja"},{"creatorName":"フジワラ, ユウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujiwara, Yuichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"藤原","familyNameLang":"ja"},{"familyName":"フジワラ","familyNameLang":"ja-Kana"},{"familyName":"Fujiwara","familyNameLang":"en"}],"givenNames":[{"givenName":"祐一郎","givenNameLang":"ja"},{"givenName":"ユウイチロウ","givenNameLang":"ja-Kana"},{"givenName":"Yuichiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4405","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000020532980","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000020532980"},{"nameIdentifier":"20532980","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20532980"},{"nameIdentifier":"Yuichiro_Fujiwara","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/Yuichiro_Fujiwara"},{"nameIdentifier":"0000-0002-9628-4073","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-9628-4073"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-15"}],"filename":"S0021925822006998","format":"text/html","licensetype":"license_0","url":{"objectType":"fulltext","url":"https://doi.org/10.1016/j.jbc.2022.102257"},"version_id":""}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"potassium channel","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gating","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphoinositides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein–lipid interaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"electrophysiology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bilayer recording","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Intermolecular functional coupling between phosphoinositides and the potassium channel KcsA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Intermolecular functional coupling between phosphoinositides and the potassium channel KcsA","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["2"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-08-05"},"publish_date":"2024-08-05","publish_status":"0","recid":"2000516","relation_version_is_last":true,"title":["Intermolecular functional coupling between phosphoinositides and the potassium channel KcsA"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2025-03-21T05:07:20.563535+00:00"}