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Deformation under flow and morphological recovery of cancer cells
https://kagawa-u.repo.nii.ac.jp/records/2000733
https://kagawa-u.repo.nii.ac.jp/records/200073324bd7624-8391-44a1-90cc-55749a966d52
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
https://doi.org/10.1039/D4LC00246F
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2025-02-25 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Deformation under flow and morphological recovery of cancer cells | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 識別子 | ||||||||||||||
| 識別子 | https://doi.org/10.1039/D4LC00246F | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| アクセス権 | ||||||||||||||
| アクセス権 | open access | |||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||
| 著者 |
寺尾, 京平
× 寺尾, 京平
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | がん転移過程で重要な、がん細胞の変形と回復を1細胞ずつ計測するパチンコデバイス（Pachinko Device）と呼ぶ新たな技術を開発した。毛細血管の狭窄構造を半導体微細加工技術で再現し、循環過程のがん細胞の様々な物理的な特性を得ることが可能となった。 | |||||||||||||
| 言語 | ja | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The metastatic cascade includes a blood circulation step for cells detached from the primary tumor. This stage involves significant shear stress as well as large and fast deformation as the cells circulate through the microvasculature. These mechanical stimuli are well reproduced in microfluidic devices. However, the recovery dynamics after deformation is also pivotal to understand how a cell can pass through the multiple capillary constrictions encountered during a single hemodynamic cycle. The microfluidic system developed in this work allows single cell recovery to be studied under flow-free conditions following pressure-actuated cell deformation inside constricted microchannels. We used three breast cancer cell lines – namely MCF-7, SK-BR3 and MDA-MB231 – as cellular models representative of different cancer phenotypes. Changing the size of the constriction allows exploration of moderate to strong deformation regimes, the latter being associated with the formation of plasma membrane blebs. In the regime of moderate deformation, all cell types display a fast elastic recovery behavior followed by a slower viscoelastic regime, well described by a double exponential decay. Among the three cell types, cells of the mesenchymal phenotype, i.e. the MDA-MB231 cells, are softer and the most fluid-like, in agreement with previous studies. Our main finding here is that the fast elastic recovery regime revealed by our novel microfluidic system is under the control of cell contractility ensured by the integrity of the cell cortex. Our results suggest that the cell cortex plays a major role in the transit of circulating tumor cells by allowing their fast morphological recovery after deformation in blood capillaries. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : Lab on a chip 巻 16, p. 3930-3944, 発行日 2024-07-01 |
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| 出版者 | ||||||||||||||
| 出版者 | Royal Society of Chemistry | |||||||||||||
| 言語 | en | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 1473-0189 | |||||||||||||
| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA12002097 | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報Resource | https://creativecommons.org/licenses/by-nc/3.0/ | |||||||||||||
| 権利情報 | This article is Open Access | |||||||||||||
| PubMed番号 | ||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | PMID | |||||||||||||
| 関連識別子 | 38993177 | |||||||||||||
| 他の資源との関係 | ||||||||||||||
| 関連タイプ | hasVersion | |||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | https://pmc.ncbi.nlm.nih.gov/articles/PMC11302772/ | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
