{"created":"2025-06-19T07:56:19.360379+00:00","id":2000936,"links":{},"metadata":{"_buckets":{"deposit":"50db492c-c98d-4d11-a3db-7beee7e00ddc"},"_deposit":{"created_by":50,"id":"2000936","owner":"50","owners":[50],"pid":{"revision_id":0,"type":"depid","value":"2000936"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:02000936","sets":["2"]},"author_link":["24982","24983","24984","24985"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-04","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"749","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Plasmacytoid dendritic cells produce large amounts of type-I interferon (IFN–I) upon sensing nucleic acid components of pathogens by Toll-like receptors (TLR7 and TLR9). The transcription factor Spi-B has the DNA-binding Ets domain, and transactivates the Ifna4 promoter co-operatively with IFN regulatory factor-7 (IRF-7) for TLR7/TLR9-induced IFN-I production. Spi-B associates with IRF-7, and activates transcription by binding to the 5′-AGAA-3′ sequence, being different from 5′-GGAA-3′, known as the Ets domain recognition sequence. To understand the molecular mechanism for the co-operative transactivation of the Ifna4 promoter by Spi-B and IRF-7, we performed X-ray structural determination of the Spi-B Ets domain in complex with target DNAs, including 5′-AGAA-3′ and 5′-GGAA-3′ sequences. Furthermore, we conducted a modeling study of the complex of the Spi-B and IRF-7 with Ifna4 promoter DNA. X-ray structures showed that the binding of the Spi-B Ets domain induces a kink in DNA at the recognition sequence, and a more kinked DNA structure was observed in 5′-AGAA-3′ than 5′-GGAA-3’. A modeling study showed that the Spi-B-induced kinked DNA structure in 5′-AGAA-3′ is favorable for Spi-B and IRF-7 to approach each other for association on DNA.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isReferencedBy","subitem_relation_type_id":{"subitem_relation_type_id_text":"39892964","subitem_relation_type_select":"PMID"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1090-2104","subitem_source_identifier_type":"EISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1691372060236":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/100015075","subitem_funder_identifier_type":"Crossref Funder","subitem_funder_identifier_type_uri":"https://www.crossref.org/services/funder-registry/"},"subitem_funder_names":[{"subitem_funder_name":"香川大学","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Kagawa University","subitem_funder_name_language":"en"}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"restricted access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_16ec"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野中, 康宏","creatorNameLang":"ja"},{"creatorName":"ノナカ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Nonaka, Yasuhiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"野中","familyNameLang":"ja"},{"familyName":"ノナカ","familyNameLang":"ja-Kana"},{"familyName":"Nonaka","familyNameLang":"en"}],"givenNames":[{"givenName":"康宏","givenNameLang":"ja"},{"givenName":"ヤスヒロ","givenNameLang":"ja-Kana"},{"givenName":"Yasuhiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24982","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"read0149469","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0149469"},{"nameIdentifier":"50569217","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50569217"}]},{"creatorNames":[{"creatorName":"星野, 克明","creatorNameLang":"ja"},{"creatorName":"ホシノ, カツアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Hoshino, Katsuaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"星野","familyNameLang":"ja"},{"familyName":"ホシノ","familyNameLang":"ja-Kana"},{"familyName":"Hoshino","familyNameLang":"en"}],"givenNames":[{"givenName":"克明","givenNameLang":"ja"},{"givenName":"カツアキ","givenNameLang":"ja-Kana"},{"givenName":"Katsuaki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24983","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050324843","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050324843"},{"nameIdentifier":"7000002871","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/7000002871"},{"nameIdentifier":"50324843","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50324843"}]},{"creatorNames":[{"creatorName":"中村, 隆範","creatorNameLang":"ja"},{"creatorName":"ナカムラ, タカノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamura, Takanori","creatorNameLang":"en"}],"familyNames":[{"familyName":"中村","familyNameLang":"ja"},{"familyName":"ナカムラ","familyNameLang":"ja-Kana"},{"familyName":"Nakamura","familyNameLang":"en"}],"givenNames":[{"givenName":"隆範","givenNameLang":"ja"},{"givenName":"タカノリ","givenNameLang":"ja-Kana"},{"givenName":"Takanori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24984","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70183887","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70183887"},{"nameIdentifier":"read0017444","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0017444"}]},{"creatorNames":[{"creatorName":"神鳥, 成弘","creatorNameLang":"ja"},{"creatorName":"カミトリ, シゲヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Kamitori, Shigehiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"神鳥","familyNameLang":"ja"},{"familyName":"カミトリ","familyNameLang":"ja-Kana"},{"familyName":"Kamitori","familyNameLang":"en"}],"givenNames":[{"givenName":"成弘","givenNameLang":"ja"},{"givenName":"シゲヒロ","givenNameLang":"ja-Kana"},{"givenName":"Shigehiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24985","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"kamitori","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/kamitori"},{"nameIdentifier":"00262246","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00262246"},{"nameIdentifier":"1000000262246","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000000262246"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-06-19"}],"filename":"BBRC749_151354","format":"text/html","url":{"label":"BBRC749_151354","objectType":"fulltext","url":"https://www.sciencedirect.com/science/article/pii/S0006291X25000683"},"version_id":""}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Plasmacytoid dendritic cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Spi-B","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Type I interferon","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Type I interferon regulatory factor-7","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"X-ray structure","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Structural analysis of Spi-B DNA-binding Ets domain recognizing 5′-AGAA-3′ and 5′-GGAA-3′ sequences","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Structural analysis of Spi-B DNA-binding Ets domain recognizing 5′-AGAA-3′ and 5′-GGAA-3′ sequences","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"50","path":["2"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-06-19"},"publish_date":"2025-06-19","publish_status":"0","recid":"2000936","relation_version_is_last":true,"title":["Structural analysis of Spi-B DNA-binding Ets domain recognizing 5′-AGAA-3′ and 5′-GGAA-3′ sequences"],"weko_creator_id":"50","weko_shared_id":-1},"updated":"2025-06-19T07:56:46.464982+00:00"}