{"created":"2025-09-03T01:24:15.434197+00:00","id":2001099,"links":{},"metadata":{"_buckets":{"deposit":"8d0d97ab-3292-4306-88b0-97061c9612c2"},"_deposit":{"created_by":50,"id":"2001099","owner":"50","owners":[50],"pid":{"revision_id":0,"type":"depid","value":"2001099"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:02001099","sets":["2"]},"author_link":["25103","4404","25104","25105","4405"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-05-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicVolumeNumber":"300","bibliographic_titles":[{"bibliographic_title":"Journal Of Biological Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Sugar absorption is crucial for life and relies on glucose transporters, including sodium–glucose cotransporters (SGLTs). Although the structure of SGLTs has been resolved, the substrate selectivity of SGLTs across diverse isoforms has not been determined owing to the complex substrate-recognition processes and limited analysis methods. Therefore, this study used voltage-clamp fluorometry (VCF) to explore the substrate-binding affinities of human SGLT1 in Xenopus oocytes. VCF analysis revealed high-affinity binding of D-glucose and D-galactose, which are known transported substrates. D-fructose, which is not a transported substrate, also bound to SGLT1, suggesting potential recognition despite the lack of transport activity. VCF analysis using the T287N mutant of the substrate-binding pocket, which has reduced D-glucose transport capacity, showed that its D-galactose-binding affinity exceeded its D-glucose-binding affinity. This suggests that the change in the VCF signal was due to substrate binding to the binding pocket. Both D-fructose and L-sorbose showed similar binding affinities, indicating that SGLT1 preferentially binds to pyranose-form sugars, including D-fructopyranose. Electrophysiological analysis confirmed that D-fructose binding did not affect the SGLT1 transport function. The significance of the VCF assay lies in its ability to measure sugar–protein interactions in living cells, thereby bridging the gap between structural analyses and functional characterizations of sugar transporters. Our findings also provide insights into SGLT substrate selectivity and the potential for developing medicines with reduced side effects by targeting non-glucose sugars with low bioreactivity.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"38522518","subitem_relation_type_select":"PMID"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2024 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1083-351X","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1691372060236":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"19K07301","subitem_award_uri":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19K07301/"},"subitem_award_titles":[{"subitem_award_title":"希少糖を用いたナトリウム・グルコース共輸送体の糖透過性の分子構造基盤解析","subitem_award_title_language":"ja"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder","subitem_funder_identifier_type_uri":"https://www.crossref.org/services/funder-registry/"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"21H05527","subitem_award_uri":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PUBLICLY-21H05527/"},"subitem_award_titles":[{"subitem_award_title":"弱い結合による糖の選択的吸収と血糖値の維持 公募研究   サマリー","subitem_award_title_language":"ja"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder","subitem_funder_identifier_type_uri":"https://www.crossref.org/services/funder-registry/"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"watabe, erika","creatorNameLang":"en"}],"familyNames":[{"familyName":"watabe","familyNameLang":"en"}],"givenNames":[{"givenName":"erika","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"25103","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"川鍋, 陽","creatorNameLang":"ja"},{"creatorName":"カワナベ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawanabe, Akira","creatorNameLang":"en"}],"familyNames":[{"familyName":"川鍋","familyNameLang":"ja"},{"familyName":"カワナベ","familyNameLang":"ja-Kana"},{"familyName":"Kawanabe","familyNameLang":"en"}],"givenNames":[{"givenName":"陽","givenNameLang":"ja"},{"givenName":"アキラ","givenNameLang":"ja-Kana"},{"givenName":"Akira","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4404","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010707128","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000010707128"},{"nameIdentifier":"10707128","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10707128"},{"nameIdentifier":"silverstone","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/silverstone"},{"nameIdentifier":"0000-0002-1094-6209","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-1094-6209"}]},{"creatorNames":[{"creatorName":"神鳥, 和代","creatorNameLang":"ja"},{"creatorName":"カミトリ, カズヨ","creatorNameLang":"ja-Kana"},{"creatorName":"Kamitori, Kazuyo","creatorNameLang":"en"}],"familyNames":[{"familyName":"神鳥","familyNameLang":"ja"},{"familyName":"カミトリ","familyNameLang":"ja-Kana"},{"familyName":"Kamitori","familyNameLang":"en"}],"givenNames":[{"givenName":"和代","givenNameLang":"ja"},{"givenName":"カズヨ","givenNameLang":"ja-Kana"},{"givenName":"Kazuyo","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"25104","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40457338","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40457338"},{"nameIdentifier":"1000040457338","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000040457338"},{"nameIdentifier":"read0133333","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0133333"}]},{"creatorNames":[{"creatorName":"ichihara, satoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"ichihara","familyNameLang":"en"}],"givenNames":[{"givenName":"satoko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"25105","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤原, 祐一郎","creatorNameLang":"ja"},{"creatorName":"フジワラ, ユウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujiwara, Yuichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"藤原","familyNameLang":"ja"},{"familyName":"フジワラ","familyNameLang":"ja-Kana"},{"familyName":"Fujiwara","familyNameLang":"en"}],"givenNames":[{"givenName":"祐一郎","givenNameLang":"ja"},{"givenName":"ユウイチロウ","givenNameLang":"ja-Kana"},{"givenName":"Yuichiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4405","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000020532980","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000020532980"},{"nameIdentifier":"20532980","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20532980"},{"nameIdentifier":"Yuichiro_Fujiwara","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/Yuichiro_Fujiwara"},{"nameIdentifier":"0000-0002-9628-4073","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-9628-4073"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-09-02"}],"filename":"JBC300_107215","format":"text/html","url":{"label":"JBC300_107215","objectType":"fulltext","url":"https://www.sciencedirect.com/science/article/pii/S0021925824017101"},"version_id":""}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"membrane transport","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sugar transport","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"structure-function","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"substrate specificity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"voltage-clamp fluorometry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SGLT","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"substrate recognition","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sugar binding of sodium–glucose cotransporters analyzed by voltage-clamp fluorometry","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sugar binding of sodium–glucose cotransporters analyzed by voltage-clamp fluorometry","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"50","path":["2"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-09-02"},"publish_date":"2025-09-02","publish_status":"0","recid":"2001099","relation_version_is_last":true,"title":["Sugar binding of sodium–glucose cotransporters analyzed by voltage-clamp fluorometry"],"weko_creator_id":"50","weko_shared_id":-1},"updated":"2025-09-03T01:24:48.003323+00:00"}