| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-10-07 |
| タイトル |
|
|
タイトル |
Myosin-Ie drives ruffle-edge lamellipodia formation and motility in A549 invasive lung cancer cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Morishita, Haruka
川合, 克久
Noda, Ayaka
江上, 洋平
荒木, 伸一
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Lamellipodia are generally defined as thin, sheet-like cell protrusions that constitute the actin cytoskeleton-based motile apparatus, which promotes the movement of migrating cells. Recently, we identified a novel type of lamellipodia, termed ruffle-edge lamellipodia, which have α-actinin-4 (ACTN4)-enriched multilayer membrane folds at their leading edges in certain invasive cancer cell lines. In this study, the role of unconventional myosin-Ie (Myo1E) in ACTN4-enriched ruffle-edge lamellipodia was analyzed using live-cell, immunofluorescence, and scanning electron microscopy. Immunofluorescence microscopy for endogenous Myo1E and live-cell imaging of mApple-Myo1E expressing cells showed that Myo1E was localized to ACTN4-rich lamellipodia tips in A549 cells. The wound healing assay and live-cell movies showed that Myo1E siRNA knockdown significantly suppressed cell migration and ruffle-edge lamellipodia formation. Furthermore, scanning electron microscopy demonstrated that Myo1E knockdown significantly reduced ruffle-edge structures. These results suggest that Myo1E may play an important role not only in the motility of ruffle-edge lamellipodia but also in the construction of ruffle-edge structures, which are probably associated with cancer cell invasion and metastasis. |
|
言語 |
en |
| 書誌情報 |
en : Microscopy
巻 dfaf039,
発行日 2025-09-26
|
| 出版者 |
|
|
出版者 |
Oxford University Press |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2050-5701 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
© The Author(s) 2025. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. |
| 権利 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| 助成情報 |
|
|
|
識別子タイプ |
Crossref Funder |
|
|
助成機関識別子タイプURI |
https://www.crossref.org/services/funder-registry/ |
|
|
助成機関識別子 |
https://doi.org/10.13039/501100001691 |
|
|
助成機関名 |
日本学術振興会 |
|
|
言語 |
ja |
|
|
助成機関名 |
Japan Society for the Promotion of Science |
|
|
言語 |
en |
|
|
研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23K06306/ |
|
|
研究課題番号 |
23K06306 |
|
|
研究課題名 |
ライブセルと光電子相関顕微鏡で明らかにする新規タイプのラメリポディアの存在と意義 |
|
|
言語 |
ja |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
41003730 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
lamellipodia |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ruffle membrane |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
myosin-Ie |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
actinin-4 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cell migration |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cancer invasion |
Microscopy_dfaf039
.png)
