@article{oai:kagawa-u.repo.nii.ac.jp:00002178, author = {西原, 浩 and Nishihara, Hiroshi and 大西, 千尋 and Onishi, Chihiro and 馬場, 智子 and Baba, Satoko}, issue = {1}, journal = {香川大学教育学部研究報告 第Ⅱ部, Memoirs of the Faculty of Education, Kagawa University. Part II}, month = {Mar}, note = {Flocculation of Saccharomyces diastaticus IFO 1958 was studied. Cells of IFO 1958 did not flocculate even in the stationary phase without Mg²⁺ although they began to flocculate strongly 18h after inoculation in the presence of Mg²⁺. Cycloheximide completely inhibited induction of floe‐forming ability of Mg²⁺‐deficient cells. Co‐flocculation between flocculent cells and Mg²⁺‐deficient cells was investigated by treatment by proteolytic enzymes and chemical modification. Treatment of Mg²⁺‐deficient cells by proteolytic enzymes did not affect the co‐flocculation with flocculent cells. Photo‐oxidation or mercaptoethanol‐reduction of Mg²⁺‐deficient cells failed to diminish the co‐flocculation with flocculent cells while treatment of Mg²⁺‐deficient cells by periodate caused a considerable loss of the co‐flocculation. On the other hand, flocculent cells deflocculated by proteolysis or chemical modification of protein component failed to co‐flocculate with Mg²⁺‐deficient cells. These findigs suggest that Mg²⁺‐deficient cells are non‐flocculent because of lack of protein component essential for flocculation of cells of IFO 1958.}, pages = {41--50}, title = {Essential role of Mg²⁺ in flocculation of Saccharomyces diastaticus}, volume = {56}, year = {2006}, yomi = {ニシハラ, ヒロシ and オオニシ, チヒロ and ババ, サトコ} }