@article{oai:kagawa-u.repo.nii.ac.jp:00002181, author = {西原, 浩 and Nishihara, Hiroshi and 三宅, 舞 and Miyake, Mai}, issue = {2}, journal = {香川大学教育学部研究報告 第Ⅱ部, Memoirs of the Faculty of Education, Kagawa University. Part II}, month = {Sep}, note = {Cells of Saccharomyces diastaticus IFO 1958 did not flocculate 24 h after inoculation in the medium with starch as carbon source but flocculated 48 h after inoculation. Cycloheximide completely inhibited induction of floc‐forming ability of cells grown for 24 h in the medium including starch. The effect of chemical modification of cell surface protein and carbohydrate components on the floc‐forming ability of flocculent cells grown with starch for 48 h and 72 h was studied. Treatment with proteolytic enzymes destroyed the floc‐forming ability. Photo‐oxidation in the presence of methylene blue and reduction with mercaptoethanol eliminated the flocculent ability. Oxidation with sodium periodate also deprived the cells of floc‐forming ability. High concentrations of protein‐denaturants brought about reversible deflocculation of the flocculent cells. These findings suggest that cell surface protein and carbohydrate components play essential roles in flocculation of the cells grown with starch.}, pages = {75--82}, title = {Flocculation of Saccharomyces diastaticus grown with starch}, volume = {56}, year = {2006}, yomi = {ニシハラ, ヒロシ and ミヤケ, マイ} }