@article{oai:kagawa-u.repo.nii.ac.jp:00002192, author = {西原, 浩 and Nishihara, Hiroshi and 藤原, 佳代子 and Fujiwara, Kayoko}, issue = {1}, journal = {香川大学教育学部研究報告 第Ⅱ部, Memoirs of the Faculty of Education, Kagawa University. Part II}, month = {Mar}, note = {Cells of Saccharomyces cerevisiae IFO 2018 began to flocculate in the middle exponential phase, while they were dispersed in the early‐growth phase. Cycloheximide strongly inhibited induction of floc‐forming ability of the early‐phase cells when the antibiotic was added to the growing culture. A definite co‐flocculation occurred when the non‐flocculent early‐phase cells and flocculent cells were mixed. Photo‐irradiation of early‐phase cells in the presence of methylene blue did not weaken the co‐flocculation with flocculent cells. After treatment with mercaptoethanol, early‐phase cells co‐flocculated with the complete cells much more strongly. The early‐phase cells treated with periodate failed to co‐flocculate with the complete cells. On the other hand, the complete cells deflocculated by treatment with mercaptoethanol or photo‐irradiation did not co‐flocculate with early‐phase cells. Although flocculent cells deflocculated by periodate oxidation failed to co‐flocculate with early‐phase cells, mercaptoethanol treatment of the early‐phase cells promoted the co‐flocculation. It is suggested that early‐phase cells are not able to produce surface protein component essential for self‐flocculation.}, pages = {19--29}, title = {Why do flocculent yeast cells not flocculate in early growth phase?}, volume = {58}, year = {2008}, yomi = {ニシハラ, ヒロシ and フジワラ, カヨコ} }