{"created":"2023-05-15T09:41:15.148700+00:00","id":321,"links":{},"metadata":{"_buckets":{"deposit":"406e1cff-4fe2-4152-9f54-1dba4b9cd913"},"_deposit":{"created_by":11,"id":"321","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"321"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000321","sets":["13:40"]},"author_link":["920"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract\nN-Acylethanolamine acid amidase (NAAA) is a lysosomal enzyme, hydrolyzing various bioactive N-acylethanolamines with a preference for palmitoylethanolamide. Human NAAA mRNA was previously reported to consist of multiple 3′-end splice variants. However, their tissue distributions and roles have not been examined yet. In the present study, we first identified four major splice variants (tentatively referred to as a1, a2, b2, and c2) in a human prostate cancer cell line LNCaP, which were composed of exons 1–11, exons 1–10 and 12, exons 1–9 and 12, and exons 1–8 and 12, respectively. We next developed quantitative polymerase chain reaction methods to individually quantify these NAAA variants as well as collectively measure all the variants. Among various human prostate cancer cells, the total levels of NAAA mRNAs in androgen-sensitive cells like LNCaP were higher than those in androgen-insensitive cells. In all of these prostate cells and other human cells, variants a1 and b2 showed the highest and lowest expression levels, respectively, among the four variants. Interestingly, ratios of the four variants were different by cell type. Variants a1 and a2 encoded the same full-length NAAA protein, which was catalytically active, while b2 and c2 were translated to C-terminally truncated proteins. As expressed in HEK293 cells these truncated forms were detected as catalytically inactive precursor proteins, but not as mature forms. These results revealed wide distribution of multiple variants of NAAA mRNA in various human cells and suggested that the proteins from some variants are catalytically inactive.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第656号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbalip.2016.09.018","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/11123944","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"27693242","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001074678","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2016 Elsevier B.V. All rights reserved.","subitem_rights_language":"en"},{"subitem_rights":"この博士論文の本文については、次のエルゼビアの著作権ポリシーの規定により公開しています。「博士論文の場合は、エンバーゴ期間に関係なく機関リポジトリに出版社版を公開することができます。」 ","subitem_rights_language":"ja"},{"subitem_rights":"The text of this doctoral dissertation is published according to the following Elsevier copyright policy. \"In the case of doctoral dissertations, publishers can be published in institutional repositories regardless of the Embargo period.\"","subitem_rights_language":"en"}]},"item_10006_text_30":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28490"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Sakura, Yuma","creatorNameLang":"en"},{"creatorName":"佐倉, 雄馬","creatorNameLang":"ja"},{"creatorName":"サクラ, ユウマ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Sakura","familyNameLang":"en"},{"familyName":"佐倉","familyNameLang":"ja"},{"familyName":"サクラ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yuma","givenNameLang":"en"},{"givenName":"雄馬","givenNameLang":"ja"},{"givenName":"ユウマ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"920","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"8000001631115","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/8000001631115"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-04"}],"displaytype":"detail","filename":"Med_A656.pdf","filesize":[{"value":"1.7 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kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/321/files/Med_A656_result.pdf"},"version_id":"d2210304-a7c9-49c4-8ba7-08ddbdab0b95"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"N-Acylethanolamine acid amidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endocannabinoid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Prostate cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Quantitative PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Splice variant","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-07-11"},"publish_date":"2018-07-11","publish_status":"0","recid":"321","relation_version_is_last":true,"title":["A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-10-31T08:00:01.749484+00:00"}