{"created":"2023-05-15T09:41:16.210734+00:00","id":347,"links":{},"metadata":{"_buckets":{"deposit":"6894bb3d-3bae-40b1-a085-6f7daab75a89"},"_deposit":{"created_by":11,"id":"347","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"347"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000347","sets":["13:40"]},"author_link":["943"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-12-26"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract\nATP-binding cassette transporter A1 (ABCA1), a 254-kD membrane protein, is a key regulator of lipid efflux from cells to apolipoproteins. ABCA1 in pancreatic β-cells influences insulin secretion and cholesterol homeostasis. Tumor necrosis factor (TNF)-α is a pleiotropic cytokine that elicits a wide spectrum of physiological events, including cell proliferation, differentiation and apoptosis and is also known to decrease glucose-dependent insulin secretion in pancreatic islets. In the present study, we examined the role of TNF-α on ABCA1 expression in rat pancreatic islets and INS-1 cells. ABCA1 protein levels decreased in response to rising concentrations of TNF-α in pancreatic islets. Real-time polymerase chain reaction analysis showed a significant decrease in ABCA1 mRNA expression. In parallel with its effect on endogenous ABCA1 mRNA levels, TNF-α suppressed the activity of a reporter construct containing the ABCA1 promoter. This effect was abrogated by BIRB796, but not by SB203580 or LY-294002. The constitutively active form of p38 mitogen-activated protein kinase (MAPK) γ suppressed ABCA1 promoter activity but not p38-MAPK (α, β), while a dominant-negative mutant of p38-MAPK γ blocked the effect of TNF-α on ABCA1 promoter activity. BIRB796 inhibited the increased cholesterol ester content induced by TNF-α. However, BIRB796 had no effect on either the decreased insulin content or the ABCA1 suppression caused by TNF-α in INS-1 cells. We checked the influence of TNF-α of insulin secretion and glucose-stimulated insulin secretion in rat pancreatic islet and INS-1 cell. TNF-α suppressed the insulin secretion and glucose-stimulated insulin secretion in both rat pancreatic islet and INS-1 cell. In summary, TNF-α suppressed the expression of endogenous ABCA1 and suppress the insulin secretion in pancreatic islets and INS-1 cells. These findings raise the possibility that TNF-α may affect insulin secretion by controlling ABCA1 expression.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第700号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1530/jme-18-0167","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/11433658","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"30131353","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001368085","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: © 2018 Society for Endocrinology 2018","subitem_rights_language":"en"},{"subitem_rights":"Copyright © 2019 Bioscientifica Ltd","subitem_rights_language":"en"},{"subitem_rights":"エンバーゴ期間満了 / The embargo period has expired","subitem_rights_language":"ja"}]},"item_10006_text_30":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28576"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Sato, Seisuke","creatorNameLang":"en"},{"creatorName":"佐藤, 誠祐","creatorNameLang":"ja"},{"creatorName":"サトウ, セイスケ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Sato","familyNameLang":"en"},{"familyName":"佐藤","familyNameLang":"ja"},{"familyName":"サトウ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Seisuke","givenNameLang":"en"},{"givenName":"誠祐","givenNameLang":"ja"},{"givenName":"セイスケ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"943","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"8000001683550","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/8000001683550"},{"nameIdentifier":"50843504","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50843504"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-05"}],"displaytype":"detail","filename":"Med_A700 (Accepted Version).pdf","filesize":[{"value":"1.2 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kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/347/files/Med_A700_result.pdf"},"version_id":"d21c20fb-49b6-4b55-9e21-640f3c68c56b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"tumor necrosis factor-α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP-binding cassette transporter A1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"p38MAPK","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pancreatic β cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cholesterol accumulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effect of TNF-α on the expression of ABCA1 in pancreatic β-cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of TNF-α on the expression of ABCA1 in pancreatic β-cells","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-12-16"},"publish_date":"2019-12-16","publish_status":"0","recid":"347","relation_version_is_last":true,"title":["Effect of TNF-α on the expression of ABCA1 in pancreatic β-cells"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-10-31T08:00:01.976621+00:00"}