@article{oai:kagawa-u.repo.nii.ac.jp:00003519,
 author = {高村, 武二郎 and Takamura, Takejiro and 吉村, 奈津紀 and Yoshimura, Natsuki},
 issue = {112},
 journal = {香川大学農学部学術報告, Technical bulletin of Faculty of Agriculture, Kagawa University},
 month = {Feb},
 note = {Rapid determination of ploidy levels in cyclamen by using flow cytometry of DAPI‐stained nuclei was examined. A strong positive linear correlation between DAPI fluorescent intensities of nuclei extracted from the leaf blades of cyclamen plants and the ploidy levels in diploid, triploid and tetraploid plants was confirmed. Thus, the flow cyto‐metry of DAPI‐stained nuclei in cyclamen was proven to be an effective and easy tool to determine the ploidy levels within a short time. DAPI fluorescent intensities of nuclei extracted from the pollen grains of diploid plants having and without giant pollen grains were also investigated. Three peaks of 1C, 2C and 4C were observed in the plants having giant pollen grains, whereas only 1C and 2C peaks were observed in the plants without giant pollen. The results suggested that the giant pollen grains were unreduced 2n pollen grains., フローサイトメトリーを用いた、シクラメンの簡易かつ迅速な倍数性検定法の確立を試みた結果、各倍数体レベルとDAPIにより染色された細胞内核DNAの蛍光強度との間に高い相関が認められ、フローサイトメトリーがシクラメンの簡易倍数性検定に有効な手段であることが示された。また、フローサイトメトリーを用いて、巨大花粉を分離しないまたは分離するシクラメン二倍体個体の花粉分析した結果、前者では1Cと2Cのピークが認められたのに対して、後者では1Cと2Cに加えて4Cのピークが認められ、巨大花粉は非還元性の2n花粉であることが強く示唆された。},
 pages = {49--53},
 title = {フローサイトメトリーを用いたシクラメン植物体および花粉の倍数性検定},
 volume = {59},
 year = {2007},
 yomi = {タカムラ, タケジロウ and ヨシムラ, ナツキ}
}