{"created":"2023-05-15T09:43:18.793633+00:00","id":3523,"links":{},"metadata":{"_buckets":{"deposit":"7b9d02f6-9bb2-42c9-9c71-cf95de8d7a03"},"_deposit":{"created_by":11,"id":"3523","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3523"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00003523","sets":["1:58:321:413"]},"author_link":["1072","287"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"112","bibliographicPageEnd":"77","bibliographicPageStart":"71","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"香川大学農学部学術報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Technical bulletin of Faculty of Agriculture, Kagawa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A recombinant family 19 chitinase was highly purified from the cell extract of Escherichia coli JM109 transformed by plasmid carrying the gene encoding Streptomyces chitinase by column chromatogramphies on DEAE‐ and CM‐Sepharose. The bacterial growth after 24 hr cultivation in well of 96‐well microplate by measurement of absorbance at 595 nm showed that the purified chitinase half inhibited growth of Bacillus subtilis, but not that of Escherichia coli. When the yeast growth after 48 hr cultivation was monitored by measurement of dry weight, the purified chitinase inhibited 95% of Saccharomyces cerevisiae (budding fashion) growth and 25% of Schizosaccharomyces pombe (binary fashion) growth. The family 19 chitinase of the Streptomyces had similarity to plant family 19 chitinases (class IV type) in their catalytic domains. Therefore, this and previous our studies that the chitinase exhibits growth inhibitory activity of some bacteria, yeast, and fungi suggested that the plant family 19 chitinases (class IV) have a significant role in defense against certain microbial infections.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"放線菌Streptomycesのファミリー 19キチナーゼ遺伝子を挿入したプラスミドで形質転換した大腸菌から組換えキチナーゼをDEAE‐Sepharose及びCM‐Sepharoseの各クロマトグラフィーで高度に精製した。マイクロプレートウェルで24時間培養した細菌の増殖を595 nmの吸光度で測定した結果、精製した組換えキチナーゼは Bacillus subtilis の増殖を50%阻害したが、Escherichia coli の増殖は阻害しなかった。 48時間培養した酵母の増殖を乾燥菌体重量で測定した結果、組換えキチナーゼは出芽酵母 Saccharomyces cerevisiae の増殖を95%、分裂酵母Schizosaccharomyces pombe の増殖を25%阻害した。放線菌ファミリー 19キチナーゼの触媒ドメインはファミリー 19に属する植物キチナーゼクラスⅣと相同性を持っていた。したがって、放線菌ファミリー 19キチナーゼがある種の細菌、酵母、カビに対して増殖阻害を示すことから、植物ファミリー 19キチナーゼ（クラスⅣ）はある種の微生物感染に対する防御に重要な役割を持つと考えられた。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_heading_23":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"応用生物科学科","subitem_heading_headline":"生物資源利用学大講座","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"Department of Applied Biological Science","subitem_heading_headline":"Chair of Applied Bioresource Science","subitem_heading_language":"en"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"香川大学農学部","subitem_publisher_language":"ja"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110006270671","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010734711","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00038339","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0368-5128","subitem_source_identifier_type":"PISSN"}]},"item_10002_text_25":{"attribute_name":"WAID","attribute_value_mlt":[{"subitem_text_value":"1072"}]},"item_10002_text_26":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"1291"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山下, 陽輔","creatorNameLang":"ja"},{"creatorName":"ヤマシタ, ヨウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamashita, Yousuke","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"岡崎, 勝一郎","creatorNameLang":"ja"},{"creatorName":"オカザキ, カツイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Okazaki, Katsuichiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-14"}],"displaytype":"detail","filename":"AN00038339_059_L071.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00038339_059_L071","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/3523/files/AN00038339_059_L071.pdf"},"version_id":"66a860f0-e0b8-414a-bb44-373e68ab57f6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"recombinant family 19","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chitinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptomycs sp.","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"antimicrobial activity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"放線菌のファミリー19キチナーゼ遺伝子を持つ大腸菌から高度に精製した組換えキチナーゼの抗菌活性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"放線菌のファミリー19キチナーゼ遺伝子を持つ大腸菌から高度に精製した組換えキチナーゼの抗菌活性","subitem_title_language":"ja"},{"subitem_title":"Antimicrobial activity of recombinant family 19 chitinase highly purified from Escherichia coli carrying Streptomyces chitinase gene","subitem_title_language":"en"},{"subitem_title":"ホウシャキン ノ ファミリー 19 キチナーゼ イデンシ オ モツ ダイチョウキン カラ コウド ニ セイセイ シタ クミカエ キチナーゼ ノ コウキン カッセイ","subitem_title_language":"ja-Kana"}]},"item_type_id":"10002","owner":"11","path":["413"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-03-27"},"publish_date":"2012-03-27","publish_status":"0","recid":"3523","relation_version_is_last":true,"title":["放線菌のファミリー19キチナーゼ遺伝子を持つ大腸菌から高度に精製した組換えキチナーゼの抗菌活性"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-17T06:04:43.385568+00:00"}