{"created":"2023-05-15T09:43:18.918164+00:00","id":3526,"links":{},"metadata":{"_buckets":{"deposit":"b6c69a29-1029-4919-9a11-cf15fdea53d9"},"_deposit":{"created_by":11,"id":"3526","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3526"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00003526","sets":["1:58:321:413"]},"author_link":["1823","772"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"112","bibliographicPageEnd":"97","bibliographicPageStart":"93","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"香川大学農学部学術報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Technical bulletin of Faculty of Agriculture, Kagawa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to establish an immunoaffinity column‐HPLC method for zearalenone (ZEN) in bovine urine, purified anti‐ZEN monoclonal antibody (ZEN.2) was bound to Affi‐Gel 10 gel, and the antibody‐binding gel was packed into a mini column. When this column was applied with 10 ml of ZEN,α‐zearalenol, β‐zearalenol, α‐zearalanol, and β‐zearalanol (100 ng/ml in PBS), these recoveries were 100%, 99.0%, 56.3%,69.9%,and 32.3%, respectively. The recoveries±SD (%) from the bovine urine spiked with 0.1, 0.5, and 2.5 ng/ml of ZEN were 99.6±9.7, 106.2±1.4, and 107.9±1.7 (n=3), respectively, in our immunoaffinity column‐HPLC method.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"抗ZENモノクローナル抗体（ZEN.2）を結合したイムノアフィニティーカラム（IAC）を作製し、ウシ尿中のゼアラレノン（ZEN）の測定法の開発を行った。作製したIACは、100ng/mlのZENとα‐ゼアラレノールとほぼ100%結合し、β‐ゼアラレノール、α‐ゼアララノール、およびβ‐ゼアララノールとそれぞれ56.3%、69.9%、および32.3%の交差反応性を示した。確立したIAC‐HPLC法で添加回収実験を行った結果、0.1、0.5、および2.5ng/mlとなるようにZENを添加した、ウシ尿からの回収率±SDは、それぞれ、99.6±9.7、106.2±1.4、および107.9±1.7（n=3）で、変動係数（CV、%）は、それぞれ、9.7、1.3、および1.6であり、0.1ng/ml添加群で若干のバラツキが認められるが、良好な値であった。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_heading_23":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"応用生物科学科","subitem_heading_headline":"生物資源利用学大講座","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"Department of Applied Biological Science","subitem_heading_headline":"Chair of Applied Bioresource Science","subitem_heading_language":"en"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"香川大学農学部","subitem_publisher_language":"ja"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110006270674","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010734714","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00038339","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0368-5128","subitem_source_identifier_type":"PISSN"}]},"item_10002_text_25":{"attribute_name":"WAID","attribute_value_mlt":[{"subitem_text_value":"1823"}]},"item_10002_text_26":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"1294"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"川村, 理","creatorNameLang":"ja"},{"creatorName":"カワムラ, オサム","creatorNameLang":"ja-Kana"},{"creatorName":"Kawamura, Osamu","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"江本, 知朗","creatorNameLang":"ja"},{"creatorName":"エモト, アキオ","creatorNameLang":"ja-Kana"},{"creatorName":"Emoto, Akio","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-14"}],"displaytype":"detail","filename":"AN00038339_059_L093.pdf","filesize":[{"value":"963.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00038339_059_L093","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/3526/files/AN00038339_059_L093.pdf"},"version_id":"8f95cbdc-6cc8-41b3-a0ca-224798afb5b0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Zearalenone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bovine urine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunoaffinity column","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"イムノアフィニティーカラム‐HPLC法によるウシ尿中のゼアラレノンの測定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イムノアフィニティーカラム‐HPLC法によるウシ尿中のゼアラレノンの測定","subitem_title_language":"ja"},{"subitem_title":"Determination of zearalenone in bovine urine by an immunoaffinity column‐HPLC method","subitem_title_language":"en"},{"subitem_title":"イムノアフィニティー カラム - HPLCホウ ニヨル ウシ ニョウチュウ ノ ゼアラレノン ノ ソクテイ","subitem_title_language":"ja-Kana"}]},"item_type_id":"10002","owner":"11","path":["413"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-03-27"},"publish_date":"2012-03-27","publish_status":"0","recid":"3526","relation_version_is_last":true,"title":["イムノアフィニティーカラム‐HPLC法によるウシ尿中のゼアラレノンの測定"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-17T06:04:52.217424+00:00"}