{"created":"2023-05-15T09:43:19.208944+00:00","id":3533,"links":{},"metadata":{"_buckets":{"deposit":"31be410b-9488-47ea-b9b4-d3ca6c5287ed"},"_deposit":{"created_by":11,"id":"3533","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3533"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00003533","sets":["1:58:321:414"]},"author_link":["336","287"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"113","bibliographicPageEnd":"52","bibliographicPageStart":"47","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"香川大学農学部学術報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Technical bulletin of Faculty of Agriculture, Kagawa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"p‐Aminobenzoic ethyl ester (ABEE)‐converted four D‐aldohexoses including rare sugar D‐allose (the C‐3 epimer of glucose) were analyzed by high‐performance liquid chromatography (HPLC) on two reverse‐phase silica columns with a 0.2 M potassium borate buffer (pH 8.9) and acetonitrile (93:7) as the eluent using a detector of fluorescence (excitation of 305 nm and emission of 360 nm). On Cosmosil AR column, the four aldohexoses, which have identical molecular weight, were eluted in the order galactose < mannose < allose < glucose, although mannose, allose, and glucose were insufficiently separated. By contrast, on XBridge column containing polyethoxysilane, the four sugars were completely separated with the sharp peak. Each sugar could be sufficiently detected up to 0.5 nmol (90 ng) with a UV (305 nm) detector and 1 pmol (0.18 ng) with a fluorescence detector. Furthermore, HPLC analysis on XBridge column revealed that rare sugar D‐altrose (C‐2 and C‐3 epimer of glucose) labeled with ABEE was eluted in the position between galactose and mannose. The HPLC analysis described here is simple, reliable, and will be applicable to the identification and quantification of traces of D‐allose and D‐altrose in biomaterials such as animal blood and plant cells.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"希少糖であるD‐アロース（グルコースのC‐3エピマー）を含む4種のアルドヘキソースをp‐アミノ安息香酸メチルエステル（ABEE）化し、2種の逆相シリカカラムを用いて0.2Mホウ酸カリウム緩衝液（pH8.9）とアセトニトリル（93:7）を溶出液とした高速液体クロマトグラフィー（HPLC）で蛍光検出器（励起波長305nm、測定波長360nm）を用いて分析した。Cosmosil AR カラムでは、分子量が同一な4種のアルドヘキソースはガラクトース、マンノース、アロース、グルコースの順に溶出したが、マンノース、アロースとグルコースの相互の分離は不完全であった。それに対してポリエトキシシランを含むXBridgeカラムを用いると、4種の糖は相互にシャープなピークとして完全に分離した。各々の糖はUV検出器（305nm）では0.5nmol（90ng）以上、蛍光検出器では1pmol（0.18ng）以上が十分検出できた。さらに、XBridgeカラムを用いたHPLC分析でABEE標識した希少糖であるD‐アルトロース（グルコースのC‐2とC‐3エピマー）はガラクトースとマンノースの間の位置に溶出した。ここで示したHPLC分析は簡単で信頼性があるので、動物血清や植物細胞のような生体試料中での微量なD‐アロースやD‐アルトロースの同定や定量に応用可能である。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_heading_23":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"応用生物科学科","subitem_heading_headline":"生物資源利用学大講座","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"Department of Applied Biological Science","subitem_heading_headline":"Chair of Applied Bioresource Science","subitem_heading_language":"en"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"香川大学農学部","subitem_publisher_language":"ja"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110006657849","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010760485","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00038339","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0368-5128","subitem_source_identifier_type":"PISSN"}]},"item_10002_text_25":{"attribute_name":"WAID","attribute_value_mlt":[{"subitem_text_value":"287"}]},"item_10002_text_26":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"1301"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岡崎, 勝一郎","creatorNameLang":"ja"},{"creatorName":"オカザキ, カツイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Okazaki, Katsuichiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Izumori, Ken","creatorNameLang":"en"},{"creatorName":"何森, 健","creatorNameLang":"ja"},{"creatorName":"イズモリ, ケン","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-14"}],"displaytype":"detail","filename":"AN00038339_060_L047.pdf","filesize":[{"value":"889.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00038339_060_L047","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/3533/files/AN00038339_060_L047.pdf"},"version_id":"9812d1a1-3c94-4c7f-8716-33a4c3a9f99a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"rare sugar","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"D‐allose","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"D‐altrose","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"p‐aminobenzoic ethyl ester","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HPLC","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ポリエトキシシランを含むXBridgeカラムを用いたp‐アミノ安息香酸メチルエステル化D‐アロースとD‐アルトロース分析のための高速液体クロマトグラフィー","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ポリエトキシシランを含むXBridgeカラムを用いたp‐アミノ安息香酸メチルエステル化D‐アロースとD‐アルトロース分析のための高速液体クロマトグラフィー","subitem_title_language":"ja"},{"subitem_title":"High‐performance liquid chromatography for analysis of p‐aminobenzoic ethyl ester‐converted D‐allose and D‐altrose on XBridge column containing polyethoxysilane","subitem_title_language":"en"},{"subitem_title":"ポリエトキシシラン オ フクム XBridge カラム オ モチイタ p-アミノ アンソクコウサン メチルエステルカ D-アロース ト D-アルトロース ブンセキ ノ タメノ コウソク エキタイ クロマトグラフィー","subitem_title_language":"ja-Kana"}]},"item_type_id":"10002","owner":"11","path":["414"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-03-27"},"publish_date":"2012-03-27","publish_status":"0","recid":"3533","relation_version_is_last":true,"title":["ポリエトキシシランを含むXBridgeカラムを用いたp‐アミノ安息香酸メチルエステル化D‐アロースとD‐アルトロース分析のための高速液体クロマトグラフィー"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-02-05T02:52:42.706420+00:00"}