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イムノアフィニティーカラム-HPLC法によるカカオ加工品中のアフラトキシンの測定
https://kagawa-u.repo.nii.ac.jp/records/3549
https://kagawa-u.repo.nii.ac.jp/records/3549c890674f-6a66-44bf-86d1-6528b82bf5b1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00038339_61_85.pdf (832.3 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2012-03-27 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | イムノアフィニティーカラム-HPLC法によるカカオ加工品中のアフラトキシンの測定 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | DETERMINATION OF AFLATOXINS IN CACAO PRODUCTS BY IMMUNOAFFINITY COLUMN-HPLC METHODS | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | jpn | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| アクセス権 | ||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||
| 著者 |
川村, 理
× 川村, 理
× 濱田, あゆみ
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | In order to establish an immunoaffinity column-HPLC method for aflatoxins (AFs) in cocoa powder and cocoa, anti-AFB1 monoclonal antibody (AF.2) was bound to Affi-Gel 10 gel (16 mg/ml), and the antibody-binding gel was packed into a mini column. Cocoa powder (10 g) spiked AFs were extracted with 50 ml of acetonitrile : water (60+40). The extact were 10-fold diluted with PBS and cleaned-up by the IAC. These recoveries of 1 and 3 ng of AFs/g were 72.3～102.0% and RSD were 3.3～6.6%. But when 10 ng AFs/g were added, these recoveries were 62.9～70.7% and RSD were 6.7～22.3%. Cocoa (10 g) spiked 6 ng of AFs/g were extracted with 50 ml of acetonitrile : water : methanol (60+40+20). The extact were 20-fold diluted with PBS and cleaned-up by the IAC. These recoveries of AFB1 and B2 were 73.4 and 82.3% and RSD were 5.5 and 3.4%, respectively. But these recoveries of AFG1 and G2 were less than 45% and RSD were about 9%. Three cocoa powder and 13 cocoa samples on the market were analyzed in this method. All cocoa powder samples were contaminated 0.40～0.94 ng of AFs/g. One cocoa samples were 0.1 ng of AFG1/g. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 書誌情報 |
ja : 香川大学農学部学術報告 en : Technical bulletin of Faculty of Agriculture, Kagawa University 巻 61, p. 85-88, 発行日 2009-02-25 |
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| 出版者 | ||||||||||||||||||
| 出版者 | 香川大学農学部 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||
| 収録物識別子 | 0368-5128 | |||||||||||||||||
| 書誌レコードID | ||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AN00038339 | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Aflatoxins | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Cacao | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Monoclonal antibody | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Immunoaffinity column | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | HPLC | |||||||||||||||||
| WAID | ||||||||||||||||||
| 値 | 1823 | |||||||||||||||||
| KEID | ||||||||||||||||||
| 値 | 1464 | |||||||||||||||||
