{"created":"2023-05-15T09:41:16.540023+00:00","id":355,"links":{},"metadata":{"_buckets":{"deposit":"6c3d0ec2-0b63-45e9-8c36-afdbff49d48d"},"_deposit":{"created_by":11,"id":"355","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"355"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000355","sets":["13:40"]},"author_link":["70"],"control_number":"355","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"N-Acylphosphatidylethanolamines (NAPEs) are a class of glycerophospholipids, which are known as precursors for different bioactive N-acylethanolamines. We previously reported that phospholipase A/acyltransferase-1 (PLAAT-1), which was originally found in mammals as a tumor suppressor, catalyzes N-acylation of phosphatidylethanolamines to form NAPEs. However, recent online database suggested the presence of an uncharacterized isoform of PLAAT-1 with an extra sequence at the N terminus. In the present study, we examined the occurrence, intracellular localization, and catalytic properties of this longer isoform, as well as the original shorter isoform from humans and mice. Our results showed that human tissues express the longer isoform but not the short isoform at all. In contrast, mice expressed both isoforms with different tissue distribution. Unlike the cytoplasmic localization of the shorter isoform, the long isoform was found in both cytoplasm and nucleus, inferring that the extra sequence harbors a nuclear localization signal. As assayed with purified proteins, neither isoform required calcium for full activity. Moreover, the overexpression of each isoform remarkably increased cellular NAPE levels. These results conclude that the new long isoform of PLAAT-1 is a calcium-independent N-acyltransferase existing in both cytoplasm and nucleus and suggest a possible formation of NAPEs in various membrane structures including nuclear membrane.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第711号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1194/jlr.m071290","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/11279133/","subitem_relation_type_select":"URI"}},{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc5087872/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"27623847","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001345360","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2016 by the American Society for Biochemistry and Molecular Biology, Inc.","subitem_rights_language":"en"}]},"item_10006_text_30":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28674"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_1689051968263":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"15K08278","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K08278/"},"subitem_award_titles":[{"subitem_award_title":"癌抑制遺伝子群PLA/ATファミリーの脂質代謝酵素としての生理機能解析","subitem_award_title_language":"ja"},{"subitem_award_title":"Biochemical and functional analysis of PLAAT family members as lipid-metabolizing enzymes","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"26350894","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26350894/"},"subitem_award_titles":[{"subitem_award_title":"食欲および肥満を制御する脂質メディエーターの新規生合成機構","subitem_award_title_language":"ja"},{"subitem_award_title":"Novel mechanisms for the biosynthesis of the lipid mediator regulating appetite and obesity","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref 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family","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phospholipases/A2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phospholipids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phospholipids/biosynthesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phospholipids/phosphatidylethanolamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phospholipase A/acyltransferase-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Comparative analyses of isoforms of the calcium-independent phosphatidylethanolamine N-acyltransferase PLAAT-1 in humans and mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparative analyses of isoforms of the calcium-independent phosphatidylethanolamine N-acyltransferase PLAAT-1 in humans and mice","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-04-24"},"publish_date":"2019-04-24","publish_status":"0","recid":"355","relation_version_is_last":true,"title":["Comparative analyses of isoforms of the calcium-independent phosphatidylethanolamine N-acyltransferase PLAAT-1 in humans and mice"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-02-05T01:37:21.630639+00:00"}