{"created":"2023-05-15T09:43:20.259686+00:00","id":3558,"links":{},"metadata":{"_buckets":{"deposit":"6e5eae67-d3f0-441f-8e44-a12faa4cf053"},"_deposit":{"created_by":11,"id":"3558","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3558"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00003558","sets":["1:58:321:416"]},"author_link":["287","847","2946","1370","355"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"115","bibliographicPageEnd":"66","bibliographicPageStart":"59","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"香川大学農学部学術報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Technical bulletin of Faculty of Agriculture, Kagawa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mycodextranase from Streptomyces sp. (strain J‐13‐3) is an α‐glucanase that cleaves α‐1,4‐bonds of alternating α‐1,3‐ and α‐1,4‐linked D‐glucan (nigeran) to produce nigerose and nigeran tetrasaccharide. The gene encoding α‐glucosidase found in downstream of the mycodextranase gene was analyzed. The deduced α‐glucosidase amino acid sequence was 537 amino acids long with a calculated molecular mass of 60, 263 Da. Comparison of the encoded amino acid sequence with those of other α‐glucosidases showed that the α‐glucosidase was a member of the glycoside hydrolase family 13 and had highly conserved four regions for catalysis and substrate binding in α‐amylase family. The gene product expressed in vitro hydrolyzed p‐nitropheny α‐glucose and maltose, but not isomaltose and nigerose. These results indicated that the gene product must be an α‐1,4‐glucosidase (EC 3.2.1.20).","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"放線菌J‐13‐3株のマイコデキストラナーゼは、α‐1,3とα‐1,4が交互に連結したD‐グルカンであるニゲラン中のα‐1,4結合を分解してニゲロースとニゲラン四糖を生産するα‐グルカナーゼである。マイコデキストラナーゼ遺伝子下流に見出されたα‐グルコシダーゼをコードする遺伝子を解析した。α‐グルコシダーゼは537残基のアミノ酸からなり、分子量は60,263と推定された。推定アミノ酸配列を他のα‐グルコシダーゼと比較した結果、α‐アミラーゼが属する糖質分解酵素ファミリー13に属し、触媒や基質との結合に関与する４つの領域が高度に保存されていた。試験管内で発現させた本遺伝子の発現産物はp‐ニトロフェニルα‐グルコースとマルトースを分解したが、イソマルトースとニゲロースは分解しなかったことから、α‐1,4‐グルコシダーゼ（EC 3.2.1.20）であると考えられた。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_heading_23":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"応用生物科学科","subitem_heading_headline":"生物資源利用学大講座","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"Department of Applied Biological Science","subitem_heading_headline":"Chair of Applied Bioresource Science","subitem_heading_language":"en"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"香川大学農学部","subitem_publisher_language":"ja"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"40017014227","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010790703","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00038339","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0368-5128","subitem_source_identifier_type":"PISSN"}]},"item_10002_text_25":{"attribute_name":"WAID","attribute_value_mlt":[{"subitem_text_value":"287"}]},"item_10002_text_26":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28595"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岡崎, 勝一郎","creatorNameLang":"ja"},{"creatorName":"オカザキ, カツイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Okazaki, Katsuichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"287","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000001958713","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000001958713"},{"nameIdentifier":"60109733","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60109733"},{"nameIdentifier":"read0171097","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0171097"}]},{"creatorNames":[{"creatorName":"高瀬, 香","creatorNameLang":"ja"},{"creatorName":"タカセ, カオル","creatorNameLang":"ja-Kana"},{"creatorName":"Takase, Kaoru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"847","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000412141770","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000412141770"}]},{"creatorNames":[{"creatorName":"林, 智香","creatorNameLang":"ja"},{"creatorName":"ハヤシ, チカ","creatorNameLang":"ja-Kana"},{"creatorName":"Hayashi, Chika","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2946","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000412141771","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000412141771"}]},{"creatorNames":[{"creatorName":"小木曽, 雄介","creatorNameLang":"ja"},{"creatorName":"オギソ, ユウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Ogiso, Yusuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1370","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000412141772","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000412141772"}]},{"creatorNames":[{"creatorName":"河上, 大輔","creatorNameLang":"ja"},{"creatorName":"カワカミ, ダイスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawakami, Daisuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"355","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000412141773","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000412141773"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-14"}],"displaytype":"detail","filename":"AN00038339_062_L059.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00038339_062_L059","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/3558/files/AN00038339_062_L059.pdf"},"version_id":"8878b82e-5be7-4f56-9221-56ecd10c2048"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Streptomyces sp.","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"α‐glucosidase gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sequencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"放線菌J‐13‐3株のマイコデキストラナーゼ遺伝子の下流に見出されたα‐グルコシダーゼ遺伝子の塩基配列と発現","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"放線菌J‐13‐3株のマイコデキストラナーゼ遺伝子の下流に見出されたα‐グルコシダーゼ遺伝子の塩基配列と発現","subitem_title_language":"ja"},{"subitem_title":"Nucleotide sequence and expression of the gene encoding α‐glucosidase found in downstream of the mycodextranase gene from Streptomyces sp. J‐13‐3","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["416"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-03-08"},"publish_date":"2019-03-08","publish_status":"0","recid":"3558","relation_version_is_last":true,"title":["放線菌J‐13‐3株のマイコデキストラナーゼ遺伝子の下流に見出されたα‐グルコシダーゼ遺伝子の塩基配列と発現"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-17T07:21:51.051307+00:00"}