{"created":"2023-05-15T09:43:22.212044+00:00","id":3604,"links":{},"metadata":{"_buckets":{"deposit":"06723b69-37dc-45fa-b769-0d33d0ce9b92"},"_deposit":{"created_by":11,"id":"3604","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3604"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00003604","sets":["1:58:321:422"]},"author_link":["753","1823"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-02-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"121","bibliographicPageEnd":"35","bibliographicPageStart":"33","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"香川大学農学部学術報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Technical bulletin of Faculty of Agriculture, Kagawa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In analyses such as the mycotoxins, the immunoaffinity column (IAC)‐HPLC method is a superior method. However, the IAC‐HPLC method needs relatively a large quantity of antibodies. It becomes the problem to cost for the production of antibodies with time. Therefore, we examined the mass culture method of the antibody‐producing hybridoma using constant temperature concussion culture machine CO₂ incubation shaker CO₂‐BR‐43FL for mammals cells (Taitec Co., LTD Japan). The method, using this machine, did not need complicated operation,was able to get a culture supernatant of antibody density at the same level as the flask culture method of 1.6 L in approximately one week, and was cheap running cost. This method is a method suitable for mass production of the monoclonal antibody.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"マイコトキシンなどの分析において，IAC‐HPLC法は，すぐれた方法であるが，比較的大量の抗体を必要とし，抗体の生産に時間とコストがかかることが問題となっている．そこで，哺乳類細胞用恒温振とう培養機CO₂インキュベーションシェーカーCO₂‐BR‐43FLを用いた抗体産生ハイブリドーマの大量培養法について検討を行った．この方法は，ランニングコストが低く，煩雑な操作を必要とせず，約１週間で1.6Lのフラスコ培養法と同程度の抗体濃度の培養上清を得ることができた．モノクローナル抗体の大量生産に適した方法である．","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"香川大学農学部","subitem_publisher_language":"ja"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"120007012797","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010901548","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00038339","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0368-5128","subitem_source_identifier_type":"PISSN"}]},"item_10002_text_25":{"attribute_name":"WAID","attribute_value_mlt":[{"subitem_text_value":"753"}]},"item_10002_text_26":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28587"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"光元, 結花","creatorNameLang":"ja"},{"creatorName":"ミツモト, ユカ","creatorNameLang":"ja-Kana"},{"creatorName":"Mitumoto, Yuka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"753","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000412141643","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000412141643"}]},{"creatorNames":[{"creatorName":"川村, 理","creatorNameLang":"ja"},{"creatorName":"カワムラ, オサム","creatorNameLang":"ja-Kana"},{"creatorName":"Kawamura, Osamu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1823","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002718050","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002718050"},{"nameIdentifier":"30204770","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30204770"},{"nameIdentifier":"read0119438","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0119438"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-14"}],"displaytype":"detail","filename":"AN00038339_068_L033.pdf","filesize":[{"value":"354.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00038339_068_L033","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/3604/files/AN00038339_068_L033.pdf"},"version_id":"53782c5e-8903-4d75-8664-131c162ec163"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"immunoaffinity column","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mass production of the monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mycotoxins","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"哺乳類細胞用恒温振とう培養機CO₂インキュベーションシェーカーCO₂-BR-43FLを用いた抗体産生ハイブリドーマの大量培養法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"哺乳類細胞用恒温振とう培養機CO₂インキュベーションシェーカーCO₂-BR-43FLを用いた抗体産生ハイブリドーマの大量培養法","subitem_title_language":"ja"},{"subitem_title":"The mass culture method of the antibody‐producing hybridoma using constant temperature concussion culture machi CO₂ incubation shaker CO₂‐BR‐43FL for mammals cells","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["422"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-03-08"},"publish_date":"2019-03-08","publish_status":"0","recid":"3604","relation_version_is_last":true,"title":["哺乳類細胞用恒温振とう培養機CO₂インキュベーションシェーカーCO₂-BR-43FLを用いた抗体産生ハイブリドーマの大量培養法"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-17T07:22:39.393361+00:00"}