{"created":"2023-05-15T09:41:16.865922+00:00","id":363,"links":{},"metadata":{"_buckets":{"deposit":"4c77c466-f313-4713-9e48-8ef1f1d1b7d4"},"_deposit":{"created_by":11,"id":"363","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"363"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000363","sets":["13:40"]},"author_link":["2420"],"control_number":"363","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)‐mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein‐labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein‐labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein‐labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein‐labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1‐mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第719号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/jemt.23140","subitem_relation_type_select":"URI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/11377989/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"30295362","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001364003","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2018 Wiley Periodicals, Inc.","subitem_rights_language":"en"}]},"item_10006_text_30":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28682"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_1689051968263":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K08788/","subitem_award_uri":"17K08788"},"subitem_award_titles":[{"subitem_award_title":"脈絡叢を介する糖の脳内輸送動態に着目した糖尿病による認知症発症促進機序の解明","subitem_award_title_language":"ja"},{"subitem_award_title":"Elucidation of the mechanism of promoting dementia caused by diabetes mellitus, focusing on the transport dynamics of glucose through the choroid plexus","subitem_award_title_language":"en"}],"subitem_funder_identifiers":{"subitem_funder_identifier":"https://doi.org/10.13039/501100001691","subitem_funder_identifier_type":"Crossref Funder"},"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Fujihara, Ryuji","creatorNameLang":"en"},{"creatorName":"藤原, 龍史","creatorNameLang":"ja"},{"creatorName":"フジハラ, リュウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Fujihara","familyNameLang":"en"},{"familyName":"藤原","familyNameLang":"ja"},{"familyName":"フジハラ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Ryuji","givenNameLang":"en"},{"givenName":"龍史","givenNameLang":"ja"},{"givenName":"リュウジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2420","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000322104725","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000322104725"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-06"}],"displaytype":"detail","filename":"Med_A719(Accepted Version).pdf","filesize":[{"value":"1.0 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kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/363/files/Med_A719_result.pdf"},"version_id":"3cde1115-0dfb-45a6-98c5-d3a4af0abad3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"BMP2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BMPR1A","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Connexin 43","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ENPP1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"histomorphometry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mineralization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ossification of the posterior longitudinal ligament","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"undecalcified frozen section","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Histomorphometry of ectopic mineralization using undecalcified frozen bone sections","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Histomorphometry of ectopic mineralization using undecalcified frozen bone sections","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-10-09"},"publish_date":"2019-10-09","publish_status":"0","recid":"363","relation_version_is_last":true,"title":["Histomorphometry of ectopic mineralization using undecalcified frozen bone sections"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-10-31T07:59:58.540573+00:00"}