{"created":"2023-05-15T09:41:17.234004+00:00","id":372,"links":{},"metadata":{"_buckets":{"deposit":"9ab8460e-ea6c-4091-bc5b-a0bcb25c7ec9"},"_deposit":{"created_by":11,"id":"372","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"372"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000372","sets":["13:40"]},"author_link":["2722"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-09-29"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract\nAlthough gemcitabine (2',2'-difluorocytidine monohydrochloride) is a common anticancer agent of cholangiocellular carcinoma (CCC), its growth inhibitory effects and gemcitabine resistance in CCC cells are poorly understood. Our aims were to uncover the mechanism underlying the antitumor effect of gemcitabine and to analyze the mechanism regulating in vitro CCC cell gemcitabine resistance. In addition, we sought to identify miRNAs associated with the antitumor effects of gemcitabine in CCCs. Using a cell proliferation assay and flow cytometry, we examined the ability of gemcitabine to inhibit cell proliferation in three types of human CCC cell lines (HuCCT-1, Huh28, TKKK). We also employed western blotting to investigate the effects of gemcitabine on cell cycle-related molecules in CCC cells. In addition, we used array chips to assess gemcitabine-mediated changes in angiogenic molecules and activated tyrosine kinase receptors in CCC cells. We used miRNA array chips to comprehensively analyze gemcitabine-induced miRNAs and examined clusters of differentially expressed miRNAs in cells with and without gemcitabine treatment. Gemcitabine inhibited cell proliferation in a dose- and time-dependent manner in HuCCT-1 cells, whereas cell proliferation was unchanged in Huh28 and TKKK cells. Gemcitabine inhibited cell cycle progression in HuCCT-1 cells from G0/G1 to S phase, resulting in G1 cell cycle arrest due to the reduction of cyclin D1 expression. In addition, gemcitabine upregulated the angiogenic molecules IL-6, IL-8, ENA-78 and MCP-1. In TKKK cells, by contrast, gemcitabine did not arrest the cell cycle or modify angiogenic molecules. Furthermore, in gemcitabine-sensitive HuCCT-1 cells, gemcitabine markedly altered miRNA expression. The miRNAs and angiogenic molecules altered by gemcitabine contribute to the inhibition of tumor growth in vitro.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第618号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3892/ijo.2015.3118","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/11337520","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"26252371","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001356414","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © Spandidos Publications 2015.","subitem_rights_language":"en"}]},"item_10006_text_30":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28736"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Toyota, Yuka","creatorNameLang":"en"},{"creatorName":"豊田, 由花","creatorNameLang":"ja"},{"creatorName":"トヨタ, ユカ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Toyota","familyNameLang":"en"},{"familyName":"豊田","familyNameLang":"ja"},{"familyName":"トヨタ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yuka","givenNameLang":"en"},{"givenName":"由花","givenNameLang":"ja"},{"givenName":"ユカ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2722","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"8000001660468","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/8000001660468"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-06"}],"displaytype":"detail","filename":"Med_A618.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/372/files/Med_A618.pdf"},"version_id":"74113241-01ae-463c-8382-3497bdb83ea8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-06"}],"displaytype":"detail","filename":"Med_A618_abstract.pdf","filesize":[{"value":"111.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/372/files/Med_A618_abstract.pdf"},"version_id":"94e5c629-4678-4395-972b-9a8da8633fb7"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-06"}],"displaytype":"detail","filename":"Med_A618_result.pdf","filesize":[{"value":"132.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/372/files/Med_A618_result.pdf"},"version_id":"2ef445cc-2187-4ff0-8fea-04c93efb9d18"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"gemcitabine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cholangiocellular carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"angiogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell cycle","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Mechanism of gemcitabine-induced suppression of human cholangiocellular carcinoma cell growth","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mechanism of gemcitabine-induced suppression of human cholangiocellular carcinoma cell growth","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-07-10"},"publish_date":"2019-07-10","publish_status":"0","recid":"372","relation_version_is_last":true,"title":["Mechanism of gemcitabine-induced suppression of human cholangiocellular carcinoma cell growth"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-10-31T08:01:35.638325+00:00"}