{"created":"2023-05-15T09:41:19.037304+00:00","id":416,"links":{},"metadata":{"_buckets":{"deposit":"c4da1223-1e02-422e-a941-d37c6c5f8dc8"},"_deposit":{"created_by":11,"id":"416","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"416"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000416","sets":["13:40"]},"author_link":["2069"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ABSTRACT\nLamellipodia are sheet-like cell protrusions driven by actin polymerization mainly through Rac1, a GTPase molecular switch. In Fcγ receptor-mediated phagocytosis of IgG-opsonized erythrocytes (IgG-Es), Rac1 activation is required for lamellipodial extension along the surface of IgG-Es. However, the significance of Rac1 deactivation in phagosome formation is poorly understood. Our live-cell imaging and electron microscopy revealed that RAW264 macrophages expressing a constitutively active Rac1 mutant showed defects in phagocytic cup formation, while lamellipodia were formed around IgG-Es. Because activated Rac1 reduced the phosphorylation levels of myosin light chains, failure of the cup formation is probably due to inhibition of actin/myosin II contractility. Reversible photo-manipulation of the Rac1 switch in macrophages fed with IgG-Es could phenocopy two lamellipodial motilities: outward-extension and cup-constriction by Rac1 ON and OFF, respectively. In conjunction with fluorescence resonance energy transfer imaging of Rac1 activity, we provide a novel mechanistic model of phagosome formation spatiotemporally controlled by Rac1 switching within a phagocytic cup.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第729号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1242/jcs.201749","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dl.ndl.go.jp/pid/11498632","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28600322","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"500001380846","subitem_relation_type_select":"NAID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017. Published by The Company of Biologists Ltd.","subitem_rights_language":"en"},{"subitem_rights":"この博士論文本文は、非営利目的でのみ使用できます。 / This doctoral thesis text, can be used only for non-commercial purposes.","subitem_rights_language":"ja"}]},"item_10006_text_30":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"28969"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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ユカ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Ikeda","familyNameLang":"en"},{"familyName":"池田","familyNameLang":"ja"},{"familyName":"イケダ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yuka","givenNameLang":"en"},{"givenName":"結香","givenNameLang":"ja"},{"givenName":"ユカ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2069","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"8000001706301","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/8000001706301"},{"nameIdentifier":"0000-0003-0363-7210","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0003-0363-7210"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-09"}],"displaytype":"detail","filename":"Med_A729.pdf","filesize":[{"value":"19.4 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imaging","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Myosin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Optogenetics","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-05-21"},"publish_date":"2020-05-21","publish_status":"0","recid":"416","relation_version_is_last":true,"title":["Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-10-31T08:00:04.169771+00:00"}