{"created":"2023-05-15T09:41:22.644097+00:00","id":514,"links":{},"metadata":{"_buckets":{"deposit":"13da5dfb-87aa-4e49-9b0d-346ba6d266b0"},"_deposit":{"created_by":11,"id":"514","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"514"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000514","sets":["2"]},"author_link":["182","3173","2204"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"127","bibliographicPageStart":"123","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"育種学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Japanese journal of breeding","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nitrate reductase (NR)-deficient mutants have been used for studying nitrate assimilation in higher plants. In this experiment, screening for nitrogen deficiency symptoms was examined to select NR- or nitrate uptake-deficient mutants in rice. Of 48,000 M2 seedlings mutagenized by gamma-ray (200 Gy), 386 seedlings exhibited pale green leaves as a symptom of nitrogen deficiency in the solution containing nitrate as a sole source of nitrogen. After determining the nitrate uptake and NADH-NR activity, 77 plants were identified as variants with a low NR activity and/or low nitrate uptake. Most of the variants were sterile, but 11 plants produced m3 seeds. In the M3 generation most of the variants exhibited wild-type levels of NR. Only two lines, NR676 and NR827, which were found to be defective in NR activity were identified as mutants with a low NR activity.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"硝酸を唯一の窒素源とする水耕液でイネの幼植物を栽培し，葉色に窒素欠乏症が認められる個体を選ぶことにより，硝酸吸収欠失ならびに硝酸還元酵素（NR）欠失突然変異体の選抜を試みた．\n品種「日本晴」の感想種子に200Gyのガンマ線を照射した後代の48,000のM2種子を用い，硝酸を唯一の窒素源とする水耕液で15日間栽培したところ386個体に窒素欠乏症が現れた．これらの幼植物について硝酸吸収とNADH（還元型ニコチン酸アミドアデニンジヌクレオチド）-NR活性の測定を行ったところ，16個体が低硝酸吸収を，54個体が低NR活性を，さらに7個体が低硝酸吸収・低NR活性を示した．これらの個体の大部分は不稔であったが，低硝酸吸収・低NR活性を示した9個体の計11個体についてはM₃種子が得られた．M₃において2個体（NR676，NR827）の後代ではNR活性が原品種の約40%で低NR活性突然変異体と認めた．NR676とNR827はM₃においてそれぞれ低硝酸吸収・低NR活性，低NR活性変異体として選抜されたものである．これら突然変異体の特徴はNR活性が従来報告されているNR欠失突然変異体と野生型の中間を示すことである．現在NR827の後代を育成して突然変異体の生化学特性を調べている．\nNR欠失突然変異体を獲得するにあたっては選抜圧の差異により，得られる突然変異体のタイプが異なることが知られている．本実験で用いた選抜方法は最も弱い選抜圧であり，このことが低NR活性突然変異体を獲得できた一員であると考えられる．","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本育種学会","subitem_publisher_language":"ja"}]},"item_10001_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"120004117396","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1270/jsbbs1951.43.123","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである","subitem_rights_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11414601","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0536-3683","subitem_source_identifier_type":"PISSN"}]},"item_10001_text_25":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"3358"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAlternatives":[{"creatorAlternative":"一井, 真比古","creatorAlternativeLang":"ja"}],"creatorNames":[{"creatorName":"一井, 眞比古","creatorNameLang":"ja"},{"creatorName":"イチイ, マサヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Ichii, Masahiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"片桐, 豊雅","creatorNameLang":"ja"},{"creatorName":"カタギリ, トヨマサ","creatorNameLang":"ja-Kana"},{"creatorName":"Katagiri, Toyomasa","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"長谷川, 博","creatorNameLang":"ja"},{"creatorName":"ハセガワ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Hasegawa, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-26"}],"displaytype":"detail","filename":"AA11414601_43_1_123.pdf","filesize":[{"value":"384.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11414601_43_1_123","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/514/files/AA11414601_43_1_123.pdf"},"version_id":"96b7f5b2-fd0a-4625-9a53-5fd8f8f6e2fb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"窒素欠乏症を指標として選抜されたイネの低硝酸還元酵素活性突然変異体","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"窒素欠乏症を指標として選抜されたイネの低硝酸還元酵素活性突然変異体","subitem_title_language":"ja"},{"subitem_title":"Mutants with low nitrate reductase activity selected from seedlings expressing nitrogen deficiency symptoms in rice (Oryza sativa L.)","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["2"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-03-27"},"publish_date":"2012-03-27","publish_status":"0","recid":"514","relation_version_is_last":true,"title":["窒素欠乏症を指標として選抜されたイネの低硝酸還元酵素活性突然変異体"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-11-16T00:33:00.437908+00:00"}