{"created":"2023-05-15T09:41:22.765809+00:00","id":517,"links":{},"metadata":{"_buckets":{"deposit":"a368fdc6-1e09-462d-ae1e-24dd1979f07f"},"_deposit":{"created_by":11,"id":"517","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"517"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00000517","sets":["2"]},"author_link":["933","2362","357","2204","175","2887","182"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"120","bibliographicPageStart":"115","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"育種学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Breeding science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It was previously reported that the rice nitrate reductase (NR)-deficient mutant M819 (wild type: cv. Norin 8) Iacks the functional heme domain of NADH-NR polypeptide possibly due to a point mutation or small deletion. In this paper we were able to locate the mutation in the NR apoenzyme gene. Northern blot analysis using a rice NADH-NR genomic clone as a probe showed that the size of NR mRNA of M819 was the same as that of the wild type, suggesting that the mutation did not involve a large deletion or insertion in the NADH-NR coding sequence. The DNA fragment containing the coding region of NADH-NR heme domain was amplified by PCR from M 819 and Norin 8. The alignment of nucleotide sequences of the domain revealed that 3 nucleotides (GTC) encoding one valine residue (Val-561 or Val-562) were deleted in M819. Thus, it was demonstrated that there was a point mutation in the coding region of NADH-NR heme dornain in the mutant M819.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"イネには2つのタイプの硝酸還元酵素(NR),即ちNADH-NR及びNAD(P)H-NRがある。イネ突然変異体M819(野生型は農林8号)ではNADH-NRの活性が欠失しており,さらにNR部分活性の測定結果からM819のNADH-NR遺伝子のヘムドメインコーディング領域には点突然変異あるいは微小な欠失が生じていると予想されている(Hasegawa et al.1992)。本実験ではその検証を試みた。イネNADH-NR遺伝子pHBH1クローンをプローブに用いてノーザンブロット解析を行ったところM819及び農林8号の両者に約3kbのパンドが検出された(図1)。約3kb以外のパンドがM819で検出されなかったことから,M819のNR遺伝子のエクソン内にはノーザンブロットで確認できるような大きな構造変異は生じていないと考えられた。次にイネ品種M201からクローニングされたNADH-NR遺伝子の塩基配列をもとにプライマーを設計し,M819及び農林8号のDNAからNADH-NRヘムドメインを含む約700bpのDNA断片をそれぞれPCRにより増幅した(図2)。得られたPCR産物はpBluescriptIISK+にクローニングした。農林8号由来のクローンを使用してPCR産物とpHBH1クローンとの相同性をサザンブロット及びノーザンブロット法により確認した後(図3),M819及び農林8号のNADH-NRヘムドメインの塩基配列を決定した(図4)。農林8号の塩基配列は既知の配列と同一であったが,19では2つ並んだバリン残基(Val-561及びVal-562)のうちどちらか一方に相当する3塩基対の欠失が認められた。またこの点突然変異は,PCR法を用いる事により容易に検出することができた(図5)。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Breeding","subitem_publisher_language":"en"},{"subitem_publisher":"日本育種学会","subitem_publisher_language":"ja"}]},"item_10001_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"120004117389","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1270/jsbbs1951.47.115","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである","subitem_rights_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11353132","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1344-7610","subitem_source_identifier_type":"PISSN"}]},"item_10001_text_25":{"attribute_name":"KEID","attribute_value_mlt":[{"subitem_text_value":"3363"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 宏之","creatorNameLang":"ja"},{"creatorName":"サトウ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Sato, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"土門, 英司","creatorNameLang":"ja"},{"creatorName":"ドモン, エイジ","creatorNameLang":"ja-Kana"},{"creatorName":"Domon, Eiji","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"河瀬, 眞琴","creatorNameLang":"ja"},{"creatorName":"カワセ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Kawase, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"長谷川, 博","creatorNameLang":"ja"},{"creatorName":"ハセガワ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Hasegawa, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"井田, 正二","creatorNameLang":"ja"},{"creatorName":"イダ, ショウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Ida, Shoji","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"矢頭, 治","creatorNameLang":"ja"},{"creatorName":"ヤトウ, オサム","creatorNameLang":"ja-Kana"},{"creatorName":"Yatou, Osamu","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorAlternatives":[{"creatorAlternative":"一井, 真比古","creatorAlternativeLang":"ja"}],"creatorNames":[{"creatorName":"一井, 眞比古","creatorNameLang":"ja"},{"creatorName":"イチイ, マサヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Ichii, Masahiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-26"}],"displaytype":"detail","filename":"AA11353132_47_2_115.pdf","filesize":[{"value":"836.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11353132_47_2_115","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/517/files/AA11353132_47_2_115.pdf"},"version_id":"b8be1852-91a1-47e8-a578-5193bd923c64"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"イネ突然変異体M819のNADH特異的硝酸還元酵素（NADH-NR）活性の低下はヘムドメインのバリン残基欠失に由来する","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イネ突然変異体M819のNADH特異的硝酸還元酵素（NADH-NR）活性の低下はヘムドメインのバリン残基欠失に由来する","subitem_title_language":"ja"},{"subitem_title":"Reduced level of NADH-dependent nitrate reductase activity in rice mutant M819 due to deletion of a valine residue in heme domain","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["2"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-03-27"},"publish_date":"2012-03-27","publish_status":"0","recid":"517","relation_version_is_last":true,"title":["イネ突然変異体M819のNADH特異的硝酸還元酵素（NADH-NR）活性の低下はヘムドメインのバリン残基欠失に由来する"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-11-16T00:33:05.069856+00:00"}