{"created":"2023-05-15T09:47:27.662523+00:00","id":9547,"links":{},"metadata":{"_buckets":{"deposit":"f89c82b6-281a-4bc2-ba2a-bfdd009b8c8b"},"_deposit":{"created_by":11,"id":"9547","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"9547"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00009547","sets":["13:40"]},"author_link":["3495"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"生理活性N-アシルエタノールアミンの生合成におけるcPLA2γ型アイソフォームの関与","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-04-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Arachidonylethanolamide (anandamide) acts as an endogenous ligand of cannabinoid receptors, while other N-acylethanolamines (NAEs), such as palmitylethanolamide and oleylethanolamide, show analgesic, anti-inflammatory, and appetite-suppressing effects through other receptors. In mammalian tissues, NAEs, including anandamide, are produced from glycerophospholipid via N-acyl-phosphatidylethanolamine (NAPE). The ɛ isoform of cytosolic phospholipase A₂ (cPLA₂) functions as an N-acyltransferase to form NAPE. Since the cPLA₂ family consists of six isoforms (α, β, γ, δ, ɛ, and ζ), the present study investigated a possible involvement of isoforms other than ɛ in the NAE biosynthesis. Firstly, when the cells overexpressing one of the cPLA₂ isoforms were labeled with [¹⁴C]ethanolamine, the increase in the production of [¹⁴C]NAPE was observed only with the ɛ-expressing cells. Secondly, when the cells co-expressing ɛ and one of the other isoforms were analyzed, the increase in [¹⁴C]N-acyl-lysophosphatidylethanolamine (lysoNAPE) and [¹⁴C]NAE was seen with the combination of ɛ and γ isoforms. Furthermore, the purified cPLA₂γ hydrolyzed not only NAPE to lysoNAPE, but also lysoNAPE to glycerophospho-N-acylethanolamine (GP-NAE). Thus, the produced GP-NAE was further hydrolyzed to NAE by glycerophosphodiesterase 1. These results suggested that cPLA₂γ is involved in the biosynthesis of NAE by its phospholipase A1/A₂ and lysophospholipase activities.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第796号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/molecules26175213","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc8433734/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"34500646","subitem_relation_type_select":"PMID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.","subitem_rights_language":"en"},{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.","subitem_rights_language":"en","subitem_rights_resource":"http://creativecommons.org/licenses/by/4.0/"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"郭, 一満","creatorNameLang":"ja"},{"creatorName":"グオ, イーマン","creatorNameLang":"ja-Kana"},{"creatorName":"Guo, Yiman","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3495","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-20"}],"displaytype":"detail","filename":"Med_A796.pdf","filesize":[{"value":"582.7 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/9547/files/Med_A796.pdf"},"version_id":"cd5678dc-d4e3-4922-8460-50738a167b9a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-20"}],"displaytype":"detail","filename":"Med_A796_abstract.pdf","filesize":[{"value":"61.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/9547/files/Med_A796_abstract.pdf"},"version_id":"54cf5615-9a2b-4fc3-b5a2-8659c5bfa652"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-20"}],"displaytype":"detail","filename":"Med_A796_result.pdf","filesize":[{"value":"116.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/9547/files/Med_A796_result.pdf"},"version_id":"147f25c3-5c13-4489-a4d2-ec4bd8e9e9c5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"N-acyltransferase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"anandamide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"endocannabinoid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phospholipase A2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Involvement of the γ isoform of cPLA₂ in the biosynthesis of bioactive N-acylethanolamines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of the γ isoform of cPLA₂ in the biosynthesis of bioactive N-acylethanolamines","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-20"},"publish_date":"2022-04-20","publish_status":"0","recid":"9547","relation_version_is_last":true,"title":["Involvement of the γ isoform of cPLA₂ in the biosynthesis of bioactive N-acylethanolamines"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-10-31T08:01:38.732746+00:00"}