{"created":"2023-05-15T09:47:27.788331+00:00","id":9550,"links":{},"metadata":{"_buckets":{"deposit":"40d78516-b20e-471d-b982-5f059057beb4"},"_deposit":{"created_by":11,"id":"9550","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"9550"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00009550","sets":["13:40"]},"author_link":["3496"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"SGLT2阻害薬であるルセオグリフロジンは、生きているマウスの腎近位尿細管におけるグルコース取り込み動態に影響を与えない","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Proximal tubules (PTs) take up most of the glucose in the glomerular filtrate and return it to peritubular capillary blood. Sodium-glucose cotransporter 2 (SGLT2) at the apical membrane takes up glucose into the cell. Glucose then flows across the cells and is transported to the interstitium via glucose transporter 2 (GLUT2) at the basolateral membrane. However, glucose transport under SGLT2 inhibition remains poorly understood. In this study, we evaluated the dynamics of a fluorescent glucose analog, 2-NBDG, in the PTs of live mice treated with or without the SGLT2 inhibitor, luseogliflozin. We employed real-time multiphoton microscopy, in which insulin enhanced 2-NBDG uptake in skeletal muscle. Influx and efflux of 2-NBDG in PT cells were compared under hypo-, normo-, and hyperglycemic conditions. Luseogliflozin did not exert significant effects on glucose influx parameters under any level of blood glucose. Our results suggest that blood glucose level per se does not alter glucose influx or efflux kinetics in PTs. In conclusion, neither SGLT2 inhibition nor blood glucose level affect glucose uptake kinetics in PTs. The former was because of glucose influx through basolateral GLUT2, which is an established bidirectional transporter.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第799号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms22158169","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc8347119/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"34360935","subitem_relation_type_select":"PMID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland.","subitem_rights_language":"en"},{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"張, 安琦","creatorNameLang":"ja"},{"creatorName":"ザン, アンチ","creatorNameLang":"ja-Kana"},{"creatorName":"Zhang, Anqi","creatorNameLang":"en"},{"creatorName":"张, 安琦","creatorNameLang":"zh-cn"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-21"}],"displaytype":"detail","filename":"Med_A799.pdf","filesize":[{"value":"3.4 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/9550/files/Med_A799.pdf"},"version_id":"16dca1a7-a020-4e7b-8789-f40416f1eec7"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-21"}],"displaytype":"detail","filename":"Med_A799_abstract.pdf","filesize":[{"value":"59.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/9550/files/Med_A799_abstract.pdf"},"version_id":"df29578e-0614-48cf-9390-a9d326ac02b1"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-21"}],"displaytype":"detail","filename":"Med_A799_result.pdf","filesize":[{"value":"108.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/9550/files/Med_A799_result.pdf"},"version_id":"aa93a8a3-8560-4524-ba62-4cccc6e1a632"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"glucose handling","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"proximal tubules","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intravital imaging","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Luseogliflozin, a SGLT2 inhibitor, does not affect glucose uptake kinetics in renal proximal tubules of live mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Luseogliflozin, a SGLT2 inhibitor, does not affect glucose uptake kinetics in renal proximal tubules of live mice","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-21"},"publish_date":"2022-04-21","publish_status":"0","recid":"9550","relation_version_is_last":true,"title":["Luseogliflozin, a SGLT2 inhibitor, does not affect glucose uptake kinetics in renal proximal tubules of live mice"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-11-24T01:45:27.376941+00:00"}