{"created":"2023-05-15T09:47:28.341548+00:00","id":9561,"links":{},"metadata":{"_buckets":{"deposit":"d83b3be7-fb4c-4763-af92-68aacd028cc8"},"_deposit":{"created_by":11,"id":"9561","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"9561"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00009561","sets":["13:40"]},"author_link":["3493"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"HDL(高比重リポタンパク質)は、CaMKK/CaMKⅣ pathwayを介してアディポネクチンの遺伝子発現を促進する","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Adiponectin (APN) is an adipokine that protects against diabetes and atherosclerosis. High-density lipoprotein (HDL) mediates reverse cholesterol transport, which also protects against atherosclerosis. In this process, the human homolog of the B class type I scavenger receptor (SR-BI/CLA-1) facilitates the cellular uptake of cholesterol from HDL. The level of circulating APN is positively correlated with the serum level of HDL-cholesterol. In this study, we investigated whether HDL stimulates the gene expression of APN through the Ca2+/calmodulin (CaM)-dependent protein kinase IV (CaMKIV) cascade. APN expression was examined using real-time PCR and western blot analysis in 3T3-L1 cells incubated with HDL. CaMKIV activity was assessed by the detection of activation loop phosphorylation (at Thr196 residue), and the effect of the constitutively active form, CaMKIVc, on APN promoter activity was investigated. Our results showed that HDL stimulated APN gene expression via hSR-BI/CLA-1. Furthermore, we explored the signaling pathways by which HDL stimulated APN expression in 3T3-L1 cells. The stimulation of APN gene expression by HDL appears to be mediated by CaMKK, as STO-609, a specific inhibitor of CaMKK2, prevents this effect. We revealed that CaMKIVc increased APN gene transcriptional activity, and the CaMKIV-dominant negative mutant blocked the effect of HDL on APN promoter activity. Finally, knockdown of hSR-BI/CLA-1 also canceled the effect of HDL on APN gene expression. These results suggest that HDL has an important role to improve the function of adipocytes by activating hSR-BI/CLA-1, and CaMKK/CaMKIV pathway is conceivable as one of the signaling pathways of this activation mechanism.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第809号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1530/jme-20-0211","subitem_relation_type_select":"DOI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"34845988","subitem_relation_type_select":"PMID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: © Society for Endocrinology 2022","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 俊博","creatorNameLang":"ja"},{"creatorName":"コバヤシ, トシヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Kobayashi, Toshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-22"}],"displaytype":"detail","filename":"Med_A809.pdf","filesize":[{"value":"602.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/9561/files/Med_A809.pdf"},"version_id":"2074c573-d568-46fb-86b0-a1014b7c6e6f"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-22"}],"displaytype":"detail","filename":"Med_A809_abstract.pdf","filesize":[{"value":"70.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/9561/files/Med_A809_abstract.pdf"},"version_id":"d0f19125-efcd-43b7-8ffb-6a7ebb8aafc2"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-22"}],"displaytype":"detail","filename":"Med_A809_result.pdf","filesize":[{"value":"85.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/9561/files/Med_A809_result.pdf"},"version_id":"bb75e496-544a-4740-a44d-a04784f0be50"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"adiponectin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HDL","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SR-BI","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CaMKK/CaMKIV pathway","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"HDL promotes adiponectin gene expression via the CAMKK/CAMKIV pathway","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"HDL promotes adiponectin gene expression via the CAMKK/CAMKIV pathway","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-22"},"publish_date":"2022-04-22","publish_status":"0","recid":"9561","relation_version_is_last":true,"title":["HDL promotes adiponectin gene expression via the CAMKK/CAMKIV pathway"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-16T02:32:00.115389+00:00"}