{"created":"2023-05-15T09:47:38.384762+00:00","id":9801,"links":{},"metadata":{"_buckets":{"deposit":"bff4f1ec-c756-4d74-9d78-90a7c9bfad37"},"_deposit":{"created_by":11,"id":"9801","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"9801"},"status":"published"},"_oai":{"id":"oai:kagawa-u.repo.nii.ac.jp:00009801","sets":["13:40"]},"author_link":["3708"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"肝細胞癌におけるMicroRNAに対するレゴラフェニブの抗腫瘍効果の検討","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-06-29"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Kagawa University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"16201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and is one of the leading causes of cancer-related deaths worldwide. Regorafenib, a multi-kinase inhibitor, is used as a second-line treatment for advanced HCC. Here, we aimed to investigate the mechanism of the antitumor effect of regorafenib on HCC and evaluate altered microRNA (miRNA) expression. Cell proliferation was examined in six HCC cell lines (HuH-7, HepG2, HLF, PLC/PRF/5, Hep3B, and Li-7) using the Cell Counting Kit-8 assay. Xenografted mouse models were used to assess the effects of regorafenib in vivo. Cell cycle analysis, western blotting analysis, and miRNA expression analysis were performed to identify the antitumor inhibitory potential of regorafenib on HCC cells. Regorafenib suppressed proliferation in HuH-7 cell and induced G0/G1 cell cycle arrest and cyclin D1 downregulation in regorafenib-sensitive cells. During miRNA analysis, miRNA molecules associated with the antitumor effect of regorafenib were found. Regorafenib suppresses cell proliferation and tumor growth in HCC by decreasing cyclin D1 via alterations in intracellular and exosomal miRNAs in HCC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第813号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms23031667","subitem_relation_type_select":"DOI"}},{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc8835935/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_22":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"35163589","subitem_relation_type_select":"PMID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 by the authors. Licensee MDPI, Basel, Switzerland.","subitem_rights_language":"en"},{"subitem_rights":"This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_fa2ee174bc00049f","subitem_version_type":"P"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"琢磨, 慧","creatorNameLang":"ja"},{"creatorName":"タクマ, ケイ","creatorNameLang":"ja-Kana"},{"creatorName":"Takuma, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-07"}],"displaytype":"detail","filename":"Med_A813.pdf","filesize":[{"value":"637.8 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://kagawa-u.repo.nii.ac.jp/record/9801/files/Med_A813.pdf"},"version_id":"c0ad9edc-9e68-4f81-ab85-bd4479e4d974"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-07"}],"displaytype":"detail","filename":"Med_A813_abstract.pdf","filesize":[{"value":"85.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨","objectType":"abstract","url":"https://kagawa-u.repo.nii.ac.jp/record/9801/files/Med_A813_abstract.pdf"},"version_id":"1e51c7d1-2804-4e90-ad0a-489f47989f99"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-07"}],"displaytype":"detail","filename":"Med_A813_result.pdf","filesize":[{"value":"171.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査の結果の要旨","objectType":"other","url":"https://kagawa-u.repo.nii.ac.jp/record/9801/files/Med_A813_result.pdf"},"version_id":"f5abe80f-8aab-404e-a34c-a86810451dd1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"hepatocellular carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"regorafenib","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell cycle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell proliferation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cyclin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"antitumor effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Antitumor effect of regorafenib on microRNA expression in hepatocellular carcinoma cell lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Antitumor effect of regorafenib on microRNA expression in hepatocellular carcinoma cell lines","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"11","path":["40"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-07"},"publish_date":"2022-07-07","publish_status":"0","recid":"9801","relation_version_is_last":true,"title":["Antitumor effect of regorafenib on microRNA expression in hepatocellular carcinoma cell lines"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-10-16T02:32:21.528138+00:00"}