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Association between microRNA‑527 and glypican‑3 in hepatocellular carcinoma
https://kagawa-u.repo.nii.ac.jp/records/158
https://kagawa-u.repo.nii.ac.jp/records/158aa69c457-2882-4d7e-b7ec-a05497514511
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文 (1.5 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2020-10-20 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Association between microRNA‑527 and glypican‑3 in hepatocellular carcinoma | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||||
| 資源タイプ | doctoral thesis | |||||||||||||
| その他（別言語等）のタイトル | ||||||||||||||
| その他のタイトル | 肝細胞癌におけるmicroRNA-527とグリピカン-3との関連性 | |||||||||||||
| 言語 | ja | |||||||||||||
| アクセス権 | ||||||||||||||
| アクセス権 | open access | |||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||
| 著者 |
野村, 圭
× 野村, 圭
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The present study aimed to identify the specific microRNAs (miRNAs/miRs) and their corresponding target genes involved in hepatocellular carcinomas (HCCs). Microarray analysis was performed to examine the miRNA expression profiles of four paired HCC and corresponding non‑cancerous (N) liver tissues using 985 miRNA probes. The Human miRNA Target database was used to identify the target genes of differentially expressed miRNAs between the HCC and N tissues. The protein expression levels of target genes in the HCC tissues and cell lines were evaluated using western blotting. miRNA‑mediated suppression of target gene expression was evaluated by transiently transfecting the miRNA into the HCC cell lines. Of the 985 miRNAs evaluated, four miRNAs were differentially expressed (three upregulated and one downregulated miRNAs). Of these four miRNAs, miRNA‑527 was highly downregulated in the HCC tissues. Glypican‑3 (GPC‑3) was predicted as a target gene of miRNA‑527. Western blotting revealed that GPC‑3 protein is highly expressed in the HCC tissues and HCC cell lines compared with N and normal cell lines. Transfection with miR‑527 resulted in suppression of GPC‑3 protein expression in the Cos7 cells. Furthermore, transfection with miR‑527 also inhibited the intrinsic expression of GPC‑3 in the Huh‑7 cell line. This indicated that miR‑527 in the HCC tissues may be an important novel miRNA that targets the GPC‑3 gene expression. GPC‑3, whose expression is regulated by miR‑527, may be involved in the development and progression of HCC. | |||||||||||||
| 言語 | en | |||||||||||||
| 学位名 | ||||||||||||||
| 言語 | ja | |||||||||||||
| 学位名 | 博士（医学） | |||||||||||||
| 学位授与機関 | ||||||||||||||
| 識別子Scheme | kakenhi | |||||||||||||
| 識別子 | 16201 | |||||||||||||
| 言語 | ja | |||||||||||||
| 機関名 | 香川大学 | |||||||||||||
| 言語 | en | |||||||||||||
| 機関名 | Kagawa University | |||||||||||||
| 学位授与年月日 | ||||||||||||||
| 学位授与年月日 | 2020-09-16 | |||||||||||||
| 学位授与番号 | ||||||||||||||
| 学位授与番号 | 乙第292号 | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | Copyright: © Nomura et al. | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報Resource | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||||||
| 権利情報 | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License. | |||||||||||||
| PubMed番号 | ||||||||||||||
| 識別子タイプ | PMID | |||||||||||||
| 関連識別子 | 33613718 | |||||||||||||
| 関連サイト | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.3892/ol.2021.12490 | |||||||||||||
| 関連サイト | ||||||||||||||
| 関連タイプ | hasVersion | |||||||||||||
| 識別子タイプ | URI | |||||||||||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7856685/ | |||||||||||||
| 他の資源との関係 | ||||||||||||||
| 関連名称 | This is the Publisher's Version of the following article: Association between microRNA-527 and glypican-3 in hepatocellular carcinoma; Oncology letters.; Volume 21 Number 3 (2021) doi: 10.3892/ol.2021.12490 , which has been published in final form at https://doi.org/10.3892/ol.2021.12490 . | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | P | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_fa2ee174bc00049f | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | hepatocellular carcinoma | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | microRNA | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | glypican 3 | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | microRNA‑527 | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | target gene | |||||||||||||
| KEID | ||||||||||||||
| 29130 | ||||||||||||||
