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Roles of Na⁺/H⁺ exchanger type 1 and intracellular pH in angiotensin II-induced reactive oxygen species generation and podocyte apoptosis
https://kagawa-u.repo.nii.ac.jp/records/200
https://kagawa-u.repo.nii.ac.jp/records/2004a70873a-e6b7-4ec7-a59c-ccc5357c40e2
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内容の要旨 (271.5 kB)
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審査の結果の要旨 (425.3 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2014-03-24 | |||||
タイトル | ||||||
タイトル | Roles of Na⁺/H⁺ exchanger type 1 and intracellular pH in angiotensin II-induced reactive oxygen species generation and podocyte apoptosis | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
著者 |
劉, 雅
× 劉, 雅 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A growing body of evidence suggests that podocyte apoptosis is a major cause of decreased podocyte number, which leads to albuminuria and glomerular injury. The aim of this study was to clarify the molecular mechanisms of angiotensin II (Ang II)-induced apoptosis in cultured mouse podocytes. We examined the effects of Ang II (100 nmol/L) on apoptosis, superoxide anions, and cytosol pH in podocytes. For intracellular pH measurements, image analysis was conducted using confocal laser microscopy after incubation with carboxy-seminaphthorhodafluor-1. Superoxide anions and intracellular pH were elevated with Ang II treatment. Apoptotic cell numbers, as measured by TUNEL staining and caspase 3 activity, were also augmented in the Ang II–treated group. Pre-treatment with olmesartan (100 nmol/L, an Ang II type 1–receptor blocker), apocynin (50 µmol/L, NADPH oxidase inhibitor), or 5-N, N hexamethylene amiloride [30 µmol/L, Na+/H+ exchanger type 1 (NHE-1) inhibitor] abolished Ang II-induced podocyte apoptosis, whereas NHE-1 mRNA and protein expression was not affected by Ang II treatment. Moreover, Ang II increased NHE-1 phosphorylation. These results suggest that superoxide production, NHE-1 activation, and intracellular alkalization were early features prior to apoptosis in Ang II– treated mouse podocytes, and may offer new insights into the mechanisms responsible for Ang II–induced podocyte injury. | |||||
言語 | en | |||||
学位名 | ||||||
言語 | ja | |||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 16201 | |||||
言語 | ja | |||||
学位授与機関名 | 香川大学 | |||||
言語 | en | |||||
学位授与機関名 | Kagawa University | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-03-24 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第585号 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2013 The Japanese Pharmacological Society | |||||
論文ID(NAID) | ||||||
識別子タイプ | NAID | |||||
関連識別子 | 10031185411 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 23800993 | |||||
関連サイト | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1254/jphs.12291FP | |||||
関連サイト | ||||||
関連タイプ | hasVersion | |||||
識別子タイプ | URI | |||||
関連識別子 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792360/ | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | angiotensin II | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Na+/H+ exchanger type 1 (NHE-1) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | intracellular pH | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | apoptosis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | podocyte | |||||
KEID | ||||||
値 | 27468 |