WEKO3
アイテム
{"_buckets": {"deposit": "476e1214-f6a8-4d84-83ba-8ccd841e4a33"}, "_deposit": {"created_by": 11, "id": "316", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "316"}, "status": "published"}, "_oai": {"id": "oai:kagawa-u.repo.nii.ac.jp:00000316", "sets": ["40"]}, "author_link": ["428"], "item_10006_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2018-06-27"}]}, "item_10006_degree_grantor_9": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_language": "ja", "subitem_degreegrantor_name": "香川大学"}, {"subitem_degreegrantor_language": "en", "subitem_degreegrantor_name": "Kagawa University"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "16201", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_10006_degree_name_8": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)", "subitem_degreename_language": "ja"}]}, "item_10006_description_7": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Abstract\nSmall-cell lung cancer (SCLC) is intractable due to its high propensity for relapse. Novel agents are thus needed for SCLC treatment. Lemongrass essential oil (LG-EO) and its major constituent, citral, have been reported to inhibit the proliferation and survival of several types of cancer cells. However, the precise mechanisms through which LG-EO and citral exert their effects on SCLC cells have not been fully elucidated. SCLC cells express Src and have high levels of Src-tyrosine kinase (Src-TK) activity. In most SCLC cell lines, constitutive phosphorylation of Stat3(Y705), which is essential for its activation, has been detected. Src-TK can phosphorylate Stat3(Y705), and activated Stat3 promotes the expression of the anti-apoptotic factors Bcl-xL and Mcl-1. In the present study, LG-EO and citral prevented Src-TK from phosphorylating Stat3(Y705), resulting in decreased Bcl-xL and Mcl-1 expression, in turn suppressing the proliferation/survival of SCLC cells. To confirm these findings, the wild-type-src gene was transfected into the LU135 SCLC cell line (LU135‑wt-src), in which Src and activated phospho-Stat3(Y705) were overexpressed. The suppression of cell proliferation and the induction of apoptosis by treatment with LG-EO or citral were significantly attenuated in the LU135-wt-src cells compared with the control LU135-mock cells. The signal transducer and activator of transcription 3 (Stat3) signaling pathway is also associated with intrinsic drug resistance. LU135-wt-src cells were significantly resistant to conventional chemotherapeutic agents compared with LU135-mock cells. The combined effects of citral and each conventional chemotherapeutic agent on SCLC cells were also evaluated. The combination treatment exerted additive or more prominent effects on LU135-wt-src, LU165 and MN1112 cells, which are relatively chemoresistant SCLC cells. These findings suggest that either LG-EO or citral, alone or in combination with chemotherapeutic agents, may be a novel therapeutic option for SCLC patients.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10006_dissertation_number_12": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第693号"}]}, "item_10006_relation_15": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.3892/ijo.2018.4314", "subitem_relation_type_select": "DOI"}}, {"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://dl.ndl.go.jp/pid/11122965", "subitem_relation_type_select": "URI"}}]}, "item_10006_relation_22": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "29568932", "subitem_relation_type_select": "PMID"}}]}, "item_10006_relation_28": {"attribute_name": "論文ID(NAID)", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "500001070097", "subitem_relation_type_select": "NAID"}}]}, "item_10006_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright © Spandidos Publications 2018.", "subitem_rights_language": "en"}]}, "item_10006_text_30": {"attribute_name": "KEID", "attribute_value_mlt": [{"subitem_text_value": "28485"}]}, "item_10006_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_fa2ee174bc00049f", "subitem_version_type": "P"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Maruoka, Takayuki", "creatorNameLang": "en"}, {"creatorName": "丸岡, 敬幸", "creatorNameLang": "ja"}, {"creatorName": "マルオカ, タカユキ", "creatorNameLang": "ja-Kana"}], "familyNames": [{"familyName": "Maruoka", "familyNameLang": "en"}, {"familyName": "丸岡", "familyNameLang": "ja"}, {"familyName": "マルオカ", "familyNameLang": "ja-Kana"}], "givenNames": [{"givenName": "Takayuki", "givenNameLang": "en"}, {"givenName": "敬幸", "givenNameLang": "ja"}, {"givenName": "タカユキ", "givenNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "428", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000003763175 ", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000003763175 "}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Med_A693.pdf", "filesize": [{"value": "716.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 716400.0, "url": {"label": "本文", "objectType": "fulltext", "url": "https://kagawa-u.repo.nii.ac.jp/record/316/files/Med_A693.pdf"}, "version_id": "a2ccdc9c-6caf-4cb1-86b8-f0a8afc5e5fb"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "Med_A693_abstract.pdf", "filesize": [{"value": "100.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 100100.0, "url": {"label": "内容の要旨", "objectType": "abstract", "url": "https://kagawa-u.repo.nii.ac.jp/record/316/files/Med_A693_abstract.pdf"}, "version_id": "3067e07d-3d58-4b79-b099-2c0da09cbc77"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "Med_A693_result.pdf", "filesize": [{"value": "88.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 88100.0, "url": {"label": "審査の結果の要旨", "objectType": "other", "url": "https://kagawa-u.repo.nii.ac.jp/record/316/files/Med_A693_result.pdf"}, "version_id": "71a9a9c5-33a8-4ef3-945b-0ff7f94105eb"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "lemongrass essential oil", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "citral", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "small-cell lung cancer", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Src", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "signal transducer and activator of transcription 3", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "chemotherapeutic agents", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "chemotherapy", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "combination therapy", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Lemongrass essential oil and citral inhibit Src/Stat3 activity and suppress the proliferation/survival of small-cell lung cancer cells, alone or in combination with chemotherapeutic agents", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Lemongrass essential oil and citral inhibit Src/Stat3 activity and suppress the proliferation/survival of small-cell lung cancer cells, alone or in combination with chemotherapeutic agents", "subitem_title_language": "en"}]}, "item_type_id": "10006", "owner": "11", "path": ["40"], "permalink_uri": "https://kagawa-u.repo.nii.ac.jp/records/316", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-07-04"}, "publish_date": "2018-07-04", "publish_status": "0", "recid": "316", "relation": {}, "relation_version_is_last": true, "title": ["Lemongrass essential oil and citral inhibit Src/Stat3 activity and suppress the proliferation/survival of small-cell lung cancer cells, alone or in combination with chemotherapeutic agents"], "weko_shared_id": -1}
Lemongrass essential oil and citral inhibit Src/Stat3 activity and suppress the proliferation/survival of small-cell lung cancer cells, alone or in combination with chemotherapeutic agents
https://kagawa-u.repo.nii.ac.jp/records/316
https://kagawa-u.repo.nii.ac.jp/records/3169c5eb759-1676-45d5-968d-0348b7c02c27
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
|
![]() |
|
|
![]() |
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2018-07-04 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Lemongrass essential oil and citral inhibit Src/Stat3 activity and suppress the proliferation/survival of small-cell lung cancer cells, alone or in combination with chemotherapeutic agents | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
丸岡, 敬幸
× 丸岡, 敬幸 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Abstract Small-cell lung cancer (SCLC) is intractable due to its high propensity for relapse. Novel agents are thus needed for SCLC treatment. Lemongrass essential oil (LG-EO) and its major constituent, citral, have been reported to inhibit the proliferation and survival of several types of cancer cells. However, the precise mechanisms through which LG-EO and citral exert their effects on SCLC cells have not been fully elucidated. SCLC cells express Src and have high levels of Src-tyrosine kinase (Src-TK) activity. In most SCLC cell lines, constitutive phosphorylation of Stat3(Y705), which is essential for its activation, has been detected. Src-TK can phosphorylate Stat3(Y705), and activated Stat3 promotes the expression of the anti-apoptotic factors Bcl-xL and Mcl-1. In the present study, LG-EO and citral prevented Src-TK from phosphorylating Stat3(Y705), resulting in decreased Bcl-xL and Mcl-1 expression, in turn suppressing the proliferation/survival of SCLC cells. To confirm these findings, the wild-type-src gene was transfected into the LU135 SCLC cell line (LU135‑wt-src), in which Src and activated phospho-Stat3(Y705) were overexpressed. The suppression of cell proliferation and the induction of apoptosis by treatment with LG-EO or citral were significantly attenuated in the LU135-wt-src cells compared with the control LU135-mock cells. The signal transducer and activator of transcription 3 (Stat3) signaling pathway is also associated with intrinsic drug resistance. LU135-wt-src cells were significantly resistant to conventional chemotherapeutic agents compared with LU135-mock cells. The combined effects of citral and each conventional chemotherapeutic agent on SCLC cells were also evaluated. The combination treatment exerted additive or more prominent effects on LU135-wt-src, LU165 and MN1112 cells, which are relatively chemoresistant SCLC cells. These findings suggest that either LG-EO or citral, alone or in combination with chemotherapeutic agents, may be a novel therapeutic option for SCLC patients. |
|||||
言語 | en | |||||
学位名 | ||||||
言語 | ja | |||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
識別子Scheme | kakenhi | |||||
識別子 | 16201 | |||||
言語 | ja | |||||
機関名 | 香川大学 | |||||
言語 | en | |||||
機関名 | Kagawa University | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2018-06-27 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第693号 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Copyright © Spandidos Publications 2018. | |||||
論文ID(NAID) | ||||||
識別子タイプ | NAID | |||||
関連識別子 | 500001070097 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 29568932 | |||||
関連サイト | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3892/ijo.2018.4314 | |||||
関連サイト | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | URI | |||||
関連識別子 | https://dl.ndl.go.jp/pid/11122965 | |||||
著者版フラグ | ||||||
出版タイプ | P | |||||
出版タイプResource | http://purl.org/coar/version/c_fa2ee174bc00049f | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | lemongrass essential oil | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | citral | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | small-cell lung cancer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Src | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | signal transducer and activator of transcription 3 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | chemotherapeutic agents | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | chemotherapy | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | combination therapy | |||||
KEID | ||||||
28485 |