WEKO3
アイテム
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Phosphorylation of Numb regulates its interaction with the clathrin-associated adaptor AP-2
名前 / ファイル | ライセンス | アクション |
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AA00642943_580_24_5791 (355.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||||||||
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公開日 | 2012-03-27 | |||||||||||||||||||||||||||||
タイトル | ||||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
タイトル | Phosphorylation of Numb regulates its interaction with the clathrin-associated adaptor AP-2 | |||||||||||||||||||||||||||||
言語 | ||||||||||||||||||||||||||||||
言語 | eng | |||||||||||||||||||||||||||||
資源タイプ | ||||||||||||||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
資源タイプ | journal article | |||||||||||||||||||||||||||||
アクセス権 | ||||||||||||||||||||||||||||||
アクセス権 | open access | |||||||||||||||||||||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||||||||||
著者 |
徳光, 浩
× 徳光, 浩
WEKO
2467
× 波多野, 直哉
WEKO
2521
× 横倉, 繁行
WEKO
267
× 末吉, 由佳× 野崎, 直仁× 小林, 良二
WEKO
1396
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抄録 | ||||||||||||||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||||||||||||||
内容記述 | Numb is thought to participate in clathrin-dependent endocytosis by directly interacting with the clathrin-associated adaptor complex AP-2, although the underlying mechanisms are unknown. Numb is also known to be phosphorylated at Ser(264)in vitro and in vivo. Here, we found that Numb is phosphorylated in vitro by Ca(2+)/calmodulin-dependent protein kinase I on Ser(283). This phosphorylation was also observed in transfected COS-7 cells, indicating its physiological relevance. Pull-down experiments showed that the phosphorylation of Numb impaired its binding to the AP-2 complex and simultaneously recruited 14-3-3 proteins in vitro. Based on experiments using Numb mutants, both the initial phosphorylation of Ser(264) and the subsequent phosphorylation of Ser(283) are sufficient to abolish the binding of Numb to AP-2 and to promote the interaction with 14-3-3 protein. These findings suggest a novel mechanism for the regulation of Numb-mediated endocytosis, namely through direct phosphorylation. | |||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
書誌情報 |
en : FEBS letters 巻 580, 号 24, p. 5797-5801, 発行日 2006-10-16 |
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出版者 | ||||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
出版者 | Elsevier | |||||||||||||||||||||||||||||
ISSN | ||||||||||||||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||||||||||||||
収録物識別子 | 0014-5793 | |||||||||||||||||||||||||||||
書誌レコードID | ||||||||||||||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||||||||||||||
収録物識別子 | AA00642943 | |||||||||||||||||||||||||||||
論文ID(NAID) | ||||||||||||||||||||||||||||||
関連タイプ | isVersionOf | |||||||||||||||||||||||||||||
識別子タイプ | NAID | |||||||||||||||||||||||||||||
関連識別子 | 120004118013 | |||||||||||||||||||||||||||||
PubMed番号 | ||||||||||||||||||||||||||||||
識別子タイプ | PMID | |||||||||||||||||||||||||||||
関連識別子 | 17022975 | |||||||||||||||||||||||||||||
権利 | ||||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
権利情報 | © 2015 Federation of European Biochemical Societies | |||||||||||||||||||||||||||||
関連サイト | ||||||||||||||||||||||||||||||
関連タイプ | isVersionOf | |||||||||||||||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||||||||||||||
関連識別子 | https://doi.org/10.1016/j.febslet.2006.09.043 | |||||||||||||||||||||||||||||
著者版フラグ | ||||||||||||||||||||||||||||||
出版タイプ | AM | |||||||||||||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||||||||
主題 | Numb | |||||||||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||||||||
主題 | Phosphorylation | |||||||||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||||||||
主題 | AP-2 complex | |||||||||||||||||||||||||||||
キーワード | ||||||||||||||||||||||||||||||
言語 | en | |||||||||||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||||||||||
主題 | Endocytosis | |||||||||||||||||||||||||||||
KEID | ||||||||||||||||||||||||||||||
4778 |