WEKO3
アイテム
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希少糖比色定量法の再確認と微生物スクリーニングへの応用
https://kagawa-u.repo.nii.ac.jp/records/7200
https://kagawa-u.repo.nii.ac.jp/records/72008cfb75ce-e8fa-4329-b9a5-ceced3197fb1
名前 / ファイル | ライセンス | アクション |
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AN00038339_071_L033 (450.7 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
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公開日 | 2021-03-22 | |||||||||||
タイトル | ||||||||||||
言語 | ja | |||||||||||
タイトル | 希少糖比色定量法の再確認と微生物スクリーニングへの応用 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Colorimetric method for rare sugars to distinguish aldoses and ketoses and its application | |||||||||||
言語 | ||||||||||||
言語 | jpn | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | departmental bulletin paper | |||||||||||
アクセス権 | ||||||||||||
アクセス権 | open access | |||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
著者 |
森本, 兼司
× 森本, 兼司
WEKO
3130
× 鈴木, 琢磨 |
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | ケトース発色試薬の1つであるSi(IV)-Mo(VI)溶液による希少糖の発色特性(SiMo法)について調べた。本試薬はD-プシコース以外のケトースに対して速やかに発色を示した。これまで問題となっていたD-フルクトースとD-プシコースの比色法による区別を可能にする方法であることがわかった。すなわち,D-プシコースを基質としてケトース3-エピメラーゼ反応により生じたD-フルクトースをSiMo法によって他検体を同時にかつ容易に分析することが可能となった。本法を用いて土壌サンプルより上記酵素を有する微生物のスクリーニングを試みたが陽性株は得られなかった。ポジティブコントロールでは発色が認められたため,本法はケトース3-エピメラーゼ活性の検出に欠かせない方法になるだろう。 | |||||||||||
言語 | ja | |||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | A rapid screening method of ketose 3-epimerase, SiMo method, was developed using Si (IV)–Mo (VI) solution. This solution is a yellow color without ketoses. Ketoses except psicose (allulose) was rapidly formed blue 12-molybdosilicate anionic species by Si (IV)–Mo (VI) under acidic pH. Although cysteine-carbazole method, which was specifically detected ketose, shows same level coloration for various ketoses, colorization by Si (IV)–Mo (VI) was different depending on the species of ketoses. By contrast, the formation rate of 12-molybdosilicate anionic species for almost aldoses was very slow. The candidates possessing ketose 3-epimerase activity was screened from various samples. This activity was detected the formation of D-fructose from D-psicose using SiMo method. Positive control such as Shinella sp. NN-6 and recombinant D-tagatose 3-epimerase was detected enzymatic activity using this method. No activity was detected by screened samples in this study, suggesting that positive colonies were not detected in SiMo method due to low detection sensitivity. | |||||||||||
言語 | en | |||||||||||
書誌情報 |
ja : 香川大学農学部学術報告 en : Technical bulletin of Faculty of Agriculture, Kagawa University 巻 71, 号 124, p. 33-36, 発行日 2019-02-28 |
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出版者 | ||||||||||||
言語 | ja | |||||||||||
出版者 | 香川大学農学部 | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 0368-5128 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AN00038339 | |||||||||||
論文ID(NAID) | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | NAID | |||||||||||
関連識別子 | 40021836983 | |||||||||||
関連サイト | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | URI | |||||||||||
関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010926819 | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | フルクトース | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | プシコース | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 発色 | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | SiMo法 | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 方法 | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 本法 | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | ケトース | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 用いて土壌サンプル | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 希少糖比色定量法 | |||||||||||
キーワード | ||||||||||||
言語 | ja | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 再確認 | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Ketose 3-epimerase | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | rapid screening | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | SiMo method |