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シトリニンに対するモノクローナル抗体の作製
https://kagawa-u.repo.nii.ac.jp/records/7218
https://kagawa-u.repo.nii.ac.jp/records/7218a0711c22-a73c-47cc-aa07-60e7b20da297
名前 / ファイル | ライセンス | アクション |
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AN00038339_073_L013 (486.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||||||
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公開日 | 2021-03-23 | |||||||||||||||||||||
タイトル | ||||||||||||||||||||||
言語 | ja | |||||||||||||||||||||
タイトル | シトリニンに対するモノクローナル抗体の作製 | |||||||||||||||||||||
タイトル | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
タイトル | Production of monoclonal antibodies against citrinin | |||||||||||||||||||||
言語 | ||||||||||||||||||||||
言語 | jpn | |||||||||||||||||||||
資源タイプ | ||||||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
資源タイプ | departmental bulletin paper | |||||||||||||||||||||
アクセス権 | ||||||||||||||||||||||
アクセス権 | open access | |||||||||||||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||
著者 |
川村, 理
× 川村, 理
× 有富, 亮哉
× 張, 燃
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抄録 | ||||||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||||||
内容記述 | シトリニン(CIT)は,腎毒性を有するマイコトキシンで,麦類やチーズやベニコウジカビ色素などを汚染している.日本ではベニコウジ色素中CIT濃度200 µg/kg以下との規格が定めされている.そこで,高感度かつ簡便なCITの測定法の開発を目的として抗CITモノクローナル抗体の作製を行った.CIT-KLHでBALB/cマウスとCB6F1マウスを各12匹をそれぞれ3回免疫した結果,それぞれのマウスで1匹ずつ2匹のマウスでCITに対する抗体価の上昇を確認した.抗体価の上昇の認められたマウスの脾臓細胞を常法で細胞融合を行った.HAT選択後,クローニングを行い安定な抗CITモノクローナル抗体産生ハイブリドーマCIT.6とCIT.7を得た.CIT.6抗体を用いた至適化した競合的間接ELISAで0.3 µg/mLまでのCITを検出可能であった.本法を用いて,食品中などのCITの高感度かつ簡易検査法の確立が期待される. | |||||||||||||||||||||
言語 | ja | |||||||||||||||||||||
抄録 | ||||||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||||||
内容記述 | Citrinin (CIT) is a nephrotoxic mycotoxin that contaminates barley, cheese, and Monascus color. In Japan, there is the regulation that the CIT concentration in Monascus color is less than 200 µg/kg. Therefore, we prepared anti-CIT monoclonal antibody for the purpose of developing a highly sensitive and simple analyzed method for CIT in these. Twelve BALB/c mice and 12 CB6F1 mice were immunized with CIT-KLH three times each, and as a result, an increase in the antibody titer against CIT was confirmed in each of the one mouse. The mouse spleen cells were fused by standard methods. After HAT selection and cloning, we obtained stable anti-CIT monoclonal antibody-producing hybridomas CIT.6 and CIT.7. Optimized competitive indirect ELISA using CIT.6 antibody was able to detect CIT up to 0.3 µg/mL. It is expected that a highly sensitive and simple analyzed method for CIT in foods will be established. | |||||||||||||||||||||
言語 | en | |||||||||||||||||||||
書誌情報 |
ja : 香川大学農学部学術報告 en : Technical bulletin of Faculty of Agriculture, Kagawa University 巻 73, 号 126, p. 13-17, 発行日 2021-02-28 |
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出版者 | ||||||||||||||||||||||
言語 | ja | |||||||||||||||||||||
出版者 | 香川大学農学部 | |||||||||||||||||||||
ISSN | ||||||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||||||
収録物識別子 | 0368-5128 | |||||||||||||||||||||
書誌レコードID | ||||||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||||||
収録物識別子 | AN00038339 | |||||||||||||||||||||
論文ID(NAID) | ||||||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||||||
識別子タイプ | NAID | |||||||||||||||||||||
関連識別子 | 120007009230 | |||||||||||||||||||||
関連サイト | ||||||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||||||
識別子タイプ | URI | |||||||||||||||||||||
関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010937294 | |||||||||||||||||||||
著者版フラグ | ||||||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | ja | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | ベニコウジ色素 | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | citrinin | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | monoclonal antibody | |||||||||||||||||||||
キーワード | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
主題Scheme | Other | |||||||||||||||||||||
主題 | Monascus color |