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Immunomodulatory effects of D-allose on cytokine production by plasmacytoid dendritic cells
https://kagawa-u.repo.nii.ac.jp/records/11576
https://kagawa-u.repo.nii.ac.jp/records/115765ef26a50-13fd-4261-a966-a9a730c11bce
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本文 (421.8 kB)
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内容の要旨 (77.9 kB)
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審査の結果の要旨 (200.8 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2023-02-03 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Immunomodulatory effects of D-allose on cytokine production by plasmacytoid dendritic cells | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| その他（別言語等）のタイトル | ||||||||||||
| その他のタイトル | 形質細胞様樹状細胞によるサイトカイン産生に対するD-アロースの免疫調整効果 | |||||||||||
| 言語 | ja | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 著者 |
髙尾, 健二郎
× 髙尾, 健二郎
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | D-Allose is classified as a 'rare sugar,' i.e., part of the group of monosaccharides that are present in low quantities in the natural world. D-Allose has been demonstrated to exert many physiological functions. The effects of the rare sugars on immune responses are largely unexplored. Here, we investigated the physiological effects of D-allose on murine dendritic cells' cytokine production. When plasmacytoid dendritic cells (pDCs) were stimulated with a Toll-like receptor 7 (TLR7) ligand, a single-stranded RNA (ssRNA), or a TLR9 ligand, CpG DNA, in the medium containing D-allose, the productions of both interferon-alpha (IFN-α) and interleukin (IL)-12p40 were severely decreased. In contrast, a normal production of these cytokines was observed when pDCs were stimulated with other TLR7 ligands, an imidazoquinoline, or a guanosine analog. In contrast to the pDCs, conventional dendritic cells (cDCs) produced IL-12p40 and tumor necrosis factor-alpha (TNF-α) in response to an imidazoquinoline or CpG DNA even though D-allose was present in the medium. D-Allose did not induce pDC death, and not inhibit the endocytic uptake of fluorophore-labeled CpG DNA into pDCs. These results suggested that D-allose exerts its inhibitory effects after CpG DNA is internalized. We analyzed the TLR7/9 signal-induced activation of downstream signaling molecules in pDCs and observed that when pDCs were stimulated with a ssRNA or CpG DNA, the phosphorylation status of the MAPK family, which includes Erk1/2, JNK/SAPK, and p38 MAPK, was attenuated in the presence of D-allose compared to D-glucose controls. The stimulation of pDCs with an imidazoquinoline induced a strong phosphorylation of these MAPK family members even in the presence of D-allose. These findings reveal that D-allose can inhibit the cytokine production by pDCs stimulated with ssRNA or CpG DNA via an attenuation of the phosphorylation of MAPK family members. | |||||||||||
| 言語 | en | |||||||||||
| 学位名 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位名 | 博士（医学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 識別子Scheme | kakenhi | |||||||||||
| 識別子 | 16201 | |||||||||||
| 言語 | ja | |||||||||||
| 機関名 | 香川大学 | |||||||||||
| 言語 | en | |||||||||||
| 機関名 | Kagawa University | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2022-12-27 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第816号 | |||||||||||
| 権利 | ||||||||||||
| 言語 | en | |||||||||||
| 権利情報 | © 2022 Elsevier Inc. All rights reserved. | |||||||||||
| 権利 | ||||||||||||
| 言語 | en | |||||||||||
| 権利情報 | This work was supported by a grant from the Japan Society for the Promotion of Science (JSPS) KAKENHI, no. JP21K08458. This work was also supported by the Program for Building Regional Innovation Ecosystems of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan; the Hyakujushi Bank Foundation for the Promotion of Science and Culture; the Fund for Kagawa University Research Promotion Program; and a Rare Sugar research grant funded by Kagawa Prefecture. | |||||||||||
| 論文ID（NAID） | ||||||||||||
| 識別子タイプ | NAID | |||||||||||
| 関連識別子 | 500001550938 | |||||||||||
| PubMed番号 | ||||||||||||
| 識別子タイプ | PMID | |||||||||||
| 関連識別子 | 36030654 | |||||||||||
| 関連サイト | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1016/j.bbrc.2022.08.037 | |||||||||||
| 関連サイト | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://dl.ndl.go.jp/pid/12691034 | |||||||||||
| 助成情報 | ||||||||||||
| 識別子タイプ | Crossref Funder | |||||||||||
| 助成機関識別子 | https://doi.org/10.13039/501100001691 | |||||||||||
| 助成機関名 | 日本学術振興会 | |||||||||||
| 言語 | ja | |||||||||||
| 助成機関名 | Japan Society for the Promotion of Science | |||||||||||
| 言語 | en | |||||||||||
| 研究課題番号URI | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K08458/ | |||||||||||
| 研究課題番号 | 21K08458 | |||||||||||
| 研究課題名 | 形質細胞様樹状細胞の活性を制御する機能性単糖の開発 | |||||||||||
| 言語 | ja | |||||||||||
| 助成情報 | ||||||||||||
| 識別子タイプ | Crossref Funder | |||||||||||
| 助成機関識別子 | https://doi.org/10.13039/501100001700 | |||||||||||
| 助成機関名 | 文部科学省 : 地域イノベーション・エコシステム形成プログラム | |||||||||||
| 言語 | ja | |||||||||||
| 助成機関名 | Ministry of Education, Culture, Sports, Science and Technology : Program for Building Regional Innovation Ecosystems | |||||||||||
| 言語 | en | |||||||||||
| 助成情報 | ||||||||||||
| 助成機関名 | 百十四銀行学術文化振興財団 | |||||||||||
| 言語 | ja | |||||||||||
| 助成機関名 | Hyakujushi Bank Foundation for the Promotion of Science and Culture | |||||||||||
| 言語 | en | |||||||||||
| 助成情報 | ||||||||||||
| 識別子タイプ | Crossref Funder | |||||||||||
| 助成機関識別子 | https://doi.org/10.13039/100015075 | |||||||||||
| 助成機関名 | 香川大学 | |||||||||||
| 言語 | ja | |||||||||||
| 助成機関名 | Kagawa University | |||||||||||
| 言語 | en | |||||||||||
| 助成情報 | ||||||||||||
| 識別子タイプ | ISNI | |||||||||||
| 助成機関識別子 | https://isni.org/isni/0000000404064650 | |||||||||||
| 助成機関名 | 香川県 | |||||||||||
| 言語 | ja | |||||||||||
| 助成機関名 | Kagawa Prefecture | |||||||||||
| 言語 | en | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | P | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_fa2ee174bc00049f | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Plasmacytoid dendritic cell | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Interferon-α | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | D-allose | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Rare sugar | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | MAPK | |||||||||||
