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Histomorphometry of ectopic mineralization using undecalcified frozen bone sections
https://kagawa-u.repo.nii.ac.jp/records/363
https://kagawa-u.repo.nii.ac.jp/records/363e4be1db8-aa6a-4795-be55-75d64b03c918
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内容の要旨 (540.7 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-10-09 | |||||
| タイトル | ||||||
| タイトル | Histomorphometry of ectopic mineralization using undecalcified frozen bone sections | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
藤原, 龍史
× 藤原, 龍史 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)‐mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein‐labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein‐labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein‐labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein‐labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1‐mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible. | |||||
| 言語 | en | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 16201 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 香川大学 | |||||
| 言語 | en | |||||
| 学位授与機関名 | Kagawa University | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2019-03-24 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第719号 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2018 Wiley Periodicals, Inc. | |||||
| 論文ID（NAID） | ||||||
| 識別子タイプ | NAID | |||||
| 関連識別子 | 500001364003 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 30295362 | |||||
| 関連サイト | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://doi.org/10.1002/jemt.23140 | |||||
| 関連サイト | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://dl.ndl.go.jp/pid/11377989/ | |||||
| 助成情報 | ||||||
| 助成機関識別子タイプ | Crossref Funder | |||||
| 助成機関識別子 | https://doi.org/10.13039/501100001691 | |||||
| 助成機関名 | 日本学術振興会 | |||||
| 言語 | ja | |||||
| 助成機関名 | Japan Society for the Promotion of Science | |||||
| 言語 | en | |||||
| 研究課題番号URI | 17K08788 | |||||
| 研究課題番号 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K08788/ | |||||
| 研究課題名 | 脈絡叢を介する糖の脳内輸送動態に着目した糖尿病による認知症発症促進機序の解明 | |||||
| 言語 | ja | |||||
| 研究課題名 | Elucidation of the mechanism of promoting dementia caused by diabetes mellitus, focusing on the transport dynamics of glucose through the choroid plexus | |||||
| 言語 | en | |||||
| 著者版フラグ | ||||||
| 出版タイプ | P | |||||
| 出版タイプResource | http://purl.org/coar/version/c_fa2ee174bc00049f | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | BMP2 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | BMPR1A | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Connexin 43 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | ENPP1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | histomorphometry | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | mineralization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | ossification of the posterior longitudinal ligament | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | undecalcified frozen section | |||||
| KEID | ||||||
| 値 | 28682 | |||||
