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Involvement of the γ isoform of cPLA₂ in the biosynthesis of bioactive N-acylethanolamines
https://kagawa-u.repo.nii.ac.jp/records/9547
https://kagawa-u.repo.nii.ac.jp/records/95470f2901a8-42c7-4fd0-9a47-774eb92c680c
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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公開日 | 2022-04-20 | |||||||||||
タイトル | ||||||||||||
タイトル | Involvement of the γ isoform of cPLA₂ in the biosynthesis of bioactive N-acylethanolamines | |||||||||||
言語 | en | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
資源タイプ | doctoral thesis | |||||||||||
その他(別言語等)のタイトル | ||||||||||||
その他のタイトル | 生理活性N-アシルエタノールアミンの生合成におけるcPLA2γ型アイソフォームの関与 | |||||||||||
言語 | ja | |||||||||||
アクセス権 | ||||||||||||
アクセス権 | open access | |||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
著者 |
郭, 一満
× 郭, 一満
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Arachidonylethanolamide (anandamide) acts as an endogenous ligand of cannabinoid receptors, while other N-acylethanolamines (NAEs), such as palmitylethanolamide and oleylethanolamide, show analgesic, anti-inflammatory, and appetite-suppressing effects through other receptors. In mammalian tissues, NAEs, including anandamide, are produced from glycerophospholipid via N-acyl-phosphatidylethanolamine (NAPE). The ɛ isoform of cytosolic phospholipase A₂ (cPLA₂) functions as an N-acyltransferase to form NAPE. Since the cPLA₂ family consists of six isoforms (α, β, γ, δ, ɛ, and ζ), the present study investigated a possible involvement of isoforms other than ɛ in the NAE biosynthesis. Firstly, when the cells overexpressing one of the cPLA₂ isoforms were labeled with [¹⁴C]ethanolamine, the increase in the production of [¹⁴C]NAPE was observed only with the ɛ-expressing cells. Secondly, when the cells co-expressing ɛ and one of the other isoforms were analyzed, the increase in [¹⁴C]N-acyl-lysophosphatidylethanolamine (lysoNAPE) and [¹⁴C]NAE was seen with the combination of ɛ and γ isoforms. Furthermore, the purified cPLA₂γ hydrolyzed not only NAPE to lysoNAPE, but also lysoNAPE to glycerophospho-N-acylethanolamine (GP-NAE). Thus, the produced GP-NAE was further hydrolyzed to NAE by glycerophosphodiesterase 1. These results suggested that cPLA₂γ is involved in the biosynthesis of NAE by its phospholipase A1/A₂ and lysophospholipase activities. | |||||||||||
言語 | en | |||||||||||
学位名 | ||||||||||||
言語 | ja | |||||||||||
学位名 | 博士(医学) | |||||||||||
学位授与機関 | ||||||||||||
識別子Scheme | kakenhi | |||||||||||
識別子 | 16201 | |||||||||||
言語 | ja | |||||||||||
機関名 | 香川大学 | |||||||||||
言語 | en | |||||||||||
機関名 | Kagawa University | |||||||||||
学位授与年月日 | ||||||||||||
学位授与年月日 | 2022-04-24 | |||||||||||
学位授与番号 | ||||||||||||
学位授与番号 | 甲第796号 | |||||||||||
権利 | ||||||||||||
言語 | en | |||||||||||
権利情報 | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | |||||||||||
権利 | ||||||||||||
言語 | en | |||||||||||
権利情報Resource | http://creativecommons.org/licenses/by/4.0/ | |||||||||||
権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | |||||||||||
PubMed番号 | ||||||||||||
識別子タイプ | PMID | |||||||||||
関連識別子 | 34500646 | |||||||||||
関連サイト | ||||||||||||
関連タイプ | isVersionOf | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | https://doi.org/10.3390/molecules26175213 | |||||||||||
関連サイト | ||||||||||||
関連タイプ | hasVersion | |||||||||||
識別子タイプ | URI | |||||||||||
関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/pmc8433734/ | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | P | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_fa2ee174bc00049f | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | N-acyltransferase | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | anandamide | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | endocannabinoid | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | phospholipase A2 |